FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843628
Sequences flagged as poor quality0
Sequence length32-301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTCATCCTCTCCCCATAGAAAAGTCCAACCTGATCGTGGTCCAGAGA212562.5195939442503095No Hit
ATTTCCCAGGGCCTCTTACCCACTCCAGGCTGTCAGCAGGGATCTTGTGA202262.3975022166168025No Hit
GGAATGTTCTATTATTAAACTCATCATGTATACTTACAGGGCTTTTGGAA136571.6188414798939819No Hit
ACCGGCATTGATTTTGGGACCCACATGCCCGAGGAATACACTCACAGGCT133401.5812656763407569No Hit
GAAGAACTTGAGGCTCGTGGGCGCTCAGCCTTCTCTTGTGTCTTTTTAGA131641.5604034005509537No Hit
AGAAGGAGAGTGTCTGTCGCTCAGTCCCACTCAGGGGCCACTCTTCTTTG101721.2057447121243012No Hit
AAACTTGCATTCGTATCCACAGATAGCAAAAGAAAAATAGGACCACAAAA97981.1614123760709698No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA97941.1609382334393832No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA96241.1407871715969597No Hit
TTTTCATTTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGAT93841.112338613701774No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT93011.1025001540963553No Hit
CACAGACCAGGTTCCAGACATGGCTATTTTTATGCCTACAGATATCATGG83010.9839644961997468No Hit
ACATAGAAAGCCAGGGTCCCAGATCAGCCACTCATTCCAGGGGAGGAAGG82640.9795786768575723No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT79210.9389209461990357No Hit
AAACAAAAGTGTTGTCTTCATGCTAGTTTAAACTAATTTTTAAAAAATTA78660.9324014850147222No Hit
TCCTATATGTGATCCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGT76410.9057309619879852No Hit
AATAACTGATGTGTTCTGTTAAGCTTATAAAGTTGAACTTTTTTTTTTTT70360.834016888960537No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT69210.8203852883024271No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC65720.7790163436965107No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA65180.7726154181700939No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC63670.7547165338277061No Hit
GGAAGAACAGGATAGAAAGACTGCTTATATATTGGTCTAAATATGTTATT61410.7279274751430725No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC61060.7237787271166912No Hit
CATCAGATAAAATCCAAGAGCTTCTTCATTTAACGTAACTCACCATATGC60170.7132290535638931No Hit
CTAAAGGCTGAATGAAAGGGTAATTCATATACTGAAGATCACACCCAAGC58660.6953301692215053No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT58450.6928409204056765No Hit
GGTGAGCAGTATTTTAAGAAGGTCCTGTTGTCAGTTTTTCAGATTTTCTT58320.6912999568530205No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG58010.6876253514582257No Hit
ACTCTGGTTTCTGGTGGGACCATTATGGACATCAGGACATTCTCACCAAG57960.6870326731687426No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA57850.6857287809318799No Hit
GTCAGTCAGCGTGTTTTGGTCGATCTGGGTCTCAGCACATTACTGATGGG55120.6533685463261057No Hit
CCTATTTCTCCTTCCTAACAGTTTACCAAAGTTGAATCATATATAAGCTT54620.6474417634312754No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA52710.6248014527730231No Hit
CAGGAAATTTCTAAATGTGACATGACCTACTTACTGTACCTGGTGACAGA52510.622430739615091No Hit
CATATAAGGCAAAGCATTAGGTACTTGGTTTATATATTAAAGATCTTACT52200.6187561342202962No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA50820.6023982134305642No Hit
AGGGGAGGAGTTCAGAGGAAGACACTTGCCAGGGAAATATCCCTGTGGCT50720.6012128568515981No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT50140.5943377886935948No Hit
GGAGGCTGGCCTGAGACATTCCTATGTCCTGCTCGCCCACAGGCCTTCTT47060.5578288060614395No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA46550.5517834875087123No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC45450.5387445651400855No Hit
TTCTTTCAGCATACCACTTCATAACTGTTCAGTTTGTACAGTTTGTTCCC44920.5324621752715651No Hit
TTGAAATAGACATTGAAGGTGTCAACCAATAAACTTCTTTAAAAACTTGT44840.5315138900083923No Hit
TTTGAGCTGTCTTGACGTTCACAGATATAAAATATTAAATATATTTTAAT43890.5202530025082145No Hit
TGAATGAAGAAGCAAATTCAAAACAGTAAACATTTCCTTTATACCTTTTT43590.5166969327713162No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC43360.5139706126396942No Hit
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG43230.5124296490870384No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT43100.5108886855343825No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG41260.48907812448140653No Hit
ATATATGGCTGTTGTGCCCTTCTCTTAGTGTTAATGAGTGCTTTTTATTT41170.488011303560337No Hit
TCTTCATGCAGCTTCCCCTAATGTTAATATCTTACATACCCATGCCTGAC41090.48706301829716414No Hit
TCCACAGCACCCAAGCTCCCCAGCCAGGCCACCCACCCCTTTGAAAGAGA40920.4850479121129218No Hit
TGGGAGCTAGCAGTGTAAACAGAGTACATACATAAAAATTACATTTTAAT40060.47485384553381343No Hit
CAGGGAGGAAAGGAGGACAGCCAACAAGTACCAGGCAGCATGGTCAACTG39840.4722460610600881No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA37940.44972428605973247No Hit
GTGTATTTATTGTAGCCGAGTATCTAATTAAACAAGTTTTTACTAAATCT37780.44782771553338674No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA36200.42909908158572263No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT35320.41866794369082105No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA33990.40290270119057214No Hit
GGATTCCATCTTAAATCCATCTTTCTCTAGAACTGAGTTTACAGTATTAA33740.39993930974315695No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG33660.398991024479984No Hit
AGCAGTATGTTGAGTTTATGGCAGATTAATCTATCATCTTTTAGAAATTT33410.3960276330325688No Hit
TCACTTCAGGGCACAGAGAGGTCACCCAGCCTTCTGCTTCTTCCCTCAGG33230.39389399119042995No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC33200.3935383842167401No Hit
GGCTACACTGGACACCTCATGGCTGTATGGCCTGGTCCAAGCCAGTGTCC32980.3909305997430147No Hit
GATACTGGTTCTACTGTTTCTAAGTATGTGATTAAAATGTACATTGTTCT32960.39069352842722144No Hit
GTACACCACACACACACACGCACACGCACACGCAGAGCTGAGGGAAGGCT31940.3786028913217674No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT31830.3772989990849047No Hit
GGGTGACAGAGAAAGATCCTATCTCAAAAAACAAAACAAGAAGAAGAAAA31170.3694756456637286No Hit
CTGAGAGCATCTGGTTGGGAAGGACTGATCTAACAGCGGCTGGCAGCTGG30020.35584404500561856No Hit
CTAAAATGCCCTCCCCTCGAACCCTTACCAAACTCCTATGCATACTACAG29760.3527621179003068No Hit
CCTGGTCTTTGAGGGGAACAACTTATATCTTACGTTTCATCTTATGTTTC29530.35003579776868476No Hit
AGTTGGGTACAGTCATGGTAATGCATTATATTTTAAGATTTTGCCTTTCT28790.3412641590843357No Hit
AGCTCTTTCTCCCTTTGCCTACTTCTCCCTCTAGTACAGAATACGCCATC28530.338182231979024No Hit
GTATGTCCAAGATCAAAGTACACTGTAAAAAGCAATACTAAACTATAATT26810.3177940988208073No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA26430.31328974382073616No Hit
CATTTCTCTTGCTTACATGAACTCTATGTCGTGGCATTCAGATCAGTCAC26420.31317120816283955No Hit
GGTGAGCACTGAGGGAATGAAAGTGGGATCAGGGATCCACTTCCTTGCCC25880.3067702826364227No Hit
CATTTTCCTGTGTCTGGCCCCCTTAGGAGGACAAAGGCCTGCCCCATGGC24780.2937313602677957No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA24730.2931386819783127No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG23290.2760695472412011No Hit
GATTCTGGCCTCTCTCCCTCTCAGGGCAGGGTGCCATTTGCCGTGCCTGA22230.26350476750416063No Hit
ATCTTGGGTGCTGTGCTCAGACTCCCCCAATCTCTTCCACAGAAATCTCC21800.25840773421460644No Hit
CATTCAAGATAGAGAAAACACTGTCTGCCAAGAATAATTGTTTTTATTTC21630.25639262803036406No Hit
AGTGCAGTTCAGTCTCCCAACCCCGCCTCTCCCTGCTGCCAACCAGGCAC20390.24169420645118464No Hit
TAGAGATCTCTATCTCTTAATGACATTTCTTTTTCCCTCAGGCTTTCTGT20310.24074592118801177No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA20300.24062738553011515No Hit
TAAGCAGTCACTACCATTGTATTCTATATCAACATGCTTTTATTTTGATA19980.23683424447742368No Hit
TAAGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTA19930.23624156618794065No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA19240.22806260579307466No Hit
ATATTTCAACAAAAAAATTATGCCTATTAGAATCAAAATATGATAGCAAA19020.22545482131934927No Hit
TATGTAAGACATTCTACTGCCATCTGCAGCATTCCAAATACTTCATGAAT18650.22106900197717474No Hit
CTAAGTAACTATCTTAAGGGTTGCTCCAAAAATCTTACCTTTTCTAGATA18220.2159719686876206No Hit
CATATGATAACAGCAAATACATGTTACTTAAAATTACCATTTCTCTCATT18160.21526075474024095No Hit
CAAACCACGCTCCTTCCACTGGGCCCAATATGACATCACAGCCCTCCCCA17970.21300857724020542No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCA17720.2100451857927902No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA17410.20637058039799533No Hit
CCCCCACTTTGACAGAAGGGCAGACTGACATCCAGTCTGGGGAGATTGGG17370.20589643776640887No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT17140.2031701176347869No Hit
TTTCCCCAAACACAGCCTTCCTTCCCCCAGATCCCCAAGCTGCTCCATCG16960.20103647579264797No Hit
CAGGATATGCCACCTTTAACTCAGTTAACTGAACTTTTGTTTTTTAATAT16650.19736187039785308No Hit
CAGATTTTTGAAAAGTACTACTATGTTCTCTAATAAAAAATAGTAGATGA16480.19534676421361072No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT16020.18989412395036676No Hit
TACCCTTATTGAGACAATGCCAACATTAATTTTAAAAACCAAAAATTCTA14910.1767366659238432No Hit
AAAAACTCACGCGACAGTAATCTGTTAAGCCATTTATTTATAATTTATAA14780.17519570237118728No Hit
GTCACTATTGGTAACAGAAAAGCTGCACTTTAGGATAACAAAGTCATACG14700.17424741710801445No Hit
TTGTTTGTTTGTTTGCTTGCTTGTTTTAAGATTGTTCCAGGACACGAAGG14260.16903184816056366No Hit
CTCCCTACAGAGTCAGCCACTTAAGCTGATAGAGACATGATGTAACCATG14030.16630552802894166No Hit
TCCTTTTAGTTTGTTAATGTGATGGAATAGTTTTCAAATTATCCTTTTTT13920.165001635792079No Hit
GACTTCTGAATGAATTTATTTCAGAGTAATTTTCCAGAACTTACTGGTTG13830.1639348148710095No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT13540.16049728079200787No Hit
GTATTCTTTACATGGCTTTTGGTCTTCTAAGTGAAGCTTTTTGTTTTTCT13530.16037874513411124No Hit
GGAGTTTCACCATATTGGCCAGGCTGTTCTCAAACTCCTGACCTTGTGAT13010.1542148909234876No Hit
TTAACCTGCATAAAATTTAATGTCTTCCAAACAAATGTAATAATTTTCAC12990.15397781960769438No Hit
CTCCCTACAGAGTCAGCCACTTAAGCTGATAGAGACATGATGTAACCGTG12930.15326660566031472No Hit
AACAGGTAGAAATAGCCCATGTCATTTAAAATTAGCACCCTGAGAAGCTC12780.15148857079186562No Hit
ACTATGAAAATGATCAGGGATATGTGAGTGTGTGTGTGTCTGTGTGTGTT12460.14769542973917413No Hit
CCTCTTCTTTATTTTCAGAGTGTCTTTTCTTTTTTGCTACTAGAGTAAAA11840.14034621894958443No Hit
CCAATACGTGTTAAAAGCAAGTTACATTTTCTCTTTTAGGAAATTAACCA11790.13975354066010137No Hit
TACTACCCAGCTAAAATTATCATCTTTGTAATTAAAGCTATAGCTTAAAA11640.13797550579165224No Hit
CACTCAAATCCTTCTAACAATACTTTATATATTTTAAATACCATTTTGAA11140.13204872289682182No Hit
TCTTGTAGGAGTTAGGCCTTGAAAGAGAGATTTAATTGTTTTATTTGTTT10580.12541072605461173No Hit
TTACAGTAAGTTTTGTTGGCTTACTTTAAAATTATTTCTCTCCTTATAAT10450.12386976250195585No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA10420.123514155528266No Hit
CCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGTAGTTTACCTAGAA9970.11818005092291864No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAA9690.1148610525018136No Hit
GTATTTCCATTTCTTAGAGGGAATGGTATATATACTTTATATAATATATA9580.1135571602649509No Hit
CATGAATGTCATATTGAGATTACATATAAGGTATCAATAAATACTCACCA9510.11272741065967463No Hit
AAGACCAAGTCACTCTTTCTATGCAAAAACACTCACTCAGTTAACTGGTG9450.112016196712295No Hit
GTGAGGGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTT9320.11047523315963909No Hit
GTGTATTACCTTAATTTGAGTGATTCTTTAGATGTATTTAGTATTTGTAA9120.10810452000170692No Hit
ACTATTCCTGCTTGACCTTCAATGCTGTTCCTCAGTTTGTCACTAAAATC9030.10703769908063743No Hit
TTTATAAGCTTAACAGAACACATCAGTTATTTTATAACTTTAAAGTGAAC8820.10454845026480865No Hit
TTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGATGACTGTA8620.10217773710687648No Hit
CTGGGGCTGTCATATAGCGCTGATCCCCCCAGTTGTGCATTTACAATGGA8490.10063677355422058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)