FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001800596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001800596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52764486
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG49517799.384681582987467RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG29939485.674172586462796RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG17041493.229727282854608RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG13210422.5036574790096506RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG11920532.2591957022001505RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC11401422.1608132409363376RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG10800342.0468957093602693RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC8589071.627812692044418RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC6512441.2342468379204907No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA6053481.1472640897136759No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG5769711.0934835980397877RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT5687641.0779295755861242RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA5304011.005223475502064RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG5031820.9536376418032386RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC4781130.9061265185071641RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA4626440.8768094509629072RNA PCR Primer, Index 1 (100% over 28bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4422800.8382153101993641RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA4277320.810643734878797RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA4153710.7872169928841911RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG4002270.7585158699356987RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3973790.7531183000626596RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG3750160.7107356262316287RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA3605770.6833706292524104RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG3364070.637563303468928RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG2864060.5428007012140703RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG2849870.5401113923482548RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG2804810.5315715574297455RNA PCR Primer, Index 1 (100% over 29bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG2341830.4438269331383234No Hit
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2262080.4287126003653291RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT2119220.4016375711496554No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGGTGCCAAGGAACTCC1965390.3724834920215086RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1938120.36731524305950786RNA PCR Primer, Index 1 (100% over 29bp)
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACAT1870240.35445052947166017No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA1829220.3466763610660398RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1825370.3459467036218262RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1824600.34580077213298355RNA PCR Primer, Index 1 (100% over 30bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA1738130.329412855457362No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1690680.32042006435919795RNA PCR Primer, Index 1 (100% over 30bp)
TACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1678960.3181988733861636RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1549310.2936274220505057RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1467360.2780961421665322RNA PCR Primer, Index 1 (100% over 30bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA1462090.2770973643143231RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCAG1459810.2766652554902174RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1399720.26527691371806406RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC1397950.26494146081514RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA1369890.2596234899360149RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1366800.2590378687665033RNA PCR Primer, Index 1 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1321080.25037294971470014No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACG1311740.24860281970717954No Hit
ATATGTGATGAATACGTGTTTGGAACTCTGAGGTGGAATTCTCGGGTGCCA1299900.2463588861644554No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1281260.24282620700597748RNA PCR Primer, Index 1 (100% over 29bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT1229380.2329938360434327No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1205670.2285002833155619RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1187800.2251135356459267RNA PCR Primer, Index 1 (100% over 29bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC1182610.22412991950684405No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1165190.22082845647354551RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1144890.21698117176769238RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1144280.2168655637051027RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1131970.21453255509776026RNA PCR Primer, Index 1 (100% over 30bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG1120250.21231136412472587RNA PCR Primer, Index 10 (97% over 36bp)
ACAGTAGTCTGCACATTGGTTATGGAATTCTCGGGTGCCAAGGAACTCCAG1083440.20533508087238828RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1069430.20267988586110738RNA PCR Primer, Index 3 (100% over 34bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTCC1068620.20252637351570146RNA PCR Primer, Index 1 (100% over 27bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1061900.20125278961307422RNA PCR Primer, Index 1 (100% over 30bp)
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC1015620.19248173856938547No Hit
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1004340.1903439370185469RNA PCR Primer, Index 1 (100% over 30bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC996700.18889599341496477No Hit
ACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA994410.1884619893767183RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT981370.18599063013709638RNA PCR Primer, Index 1 (100% over 30bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT963790.18265884367754476RNA PCR Primer, Index 1 (100% over 30bp)
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC958440.1816449041122091No Hit
GACCAATGATGAGATTGGAGGGTGTCTGAATCAAAAATTTTGATTAAAGCC952910.18059685069233877No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG950770.18019127486620454RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG950040.18005292423392505RNA PCR Primer, Index 1 (100% over 29bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC930040.1762624959522964No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAG882810.1673113995652303RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT882680.1672867617813997RNA PCR Primer, Index 1 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA879940.16676747310681658RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT866250.16417292494804175RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG866190.16416155366319687RNA PCR Primer, Index 1 (100% over 29bp)
TTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG865220.1639777178915379RNA PCR Primer, Index 10 (97% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA863970.1637408161239361No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC861640.16329923122912635RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC844490.16004893897762976No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAGG839920.1591828261152776Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCA838590.1589307626345493RNA PCR Primer, Index 48 (100% over 39bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG833700.1580040029196911RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC818980.1552142477044124RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA797560.1511546990147881No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG790440.14980530654652827No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT773500.1465948137919888RNA PCR Primer, Index 3 (100% over 34bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCCA765620.1451013850490271RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG754620.14301664949413131No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC746400.14145878347038193No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT733110.13894004387723968RNA PCR Primer, Index 1 (100% over 30bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACAT723630.1371433808717477No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG708440.13426455059185075RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA702980.13322976367096612RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA687440.13028460089614063RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGC678060.1285068900320568RNA PCR Primer, Index 48 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC662460.12555035597238642RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT660680.12521300785532147RNA PCR Primer, Index 1 (100% over 30bp)
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG657550.12461980582924659RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC642430.1217542420483353RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA638940.12109281231319112RNA PCR Primer, Index 1 (100% over 28bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC638940.12109281231319112RNA PCR Primer, Index 5 (97% over 35bp)
TACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC632270.11982870448126795RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC623120.11809458354242283RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC615580.11666559208024882RNA PCR Primer, Index 1 (100% over 31bp)
TTACATGATGAATACGTGTCTGGAACTCTGAGGTGGAATTCTCGGGTGCCA610150.11563649080178665No Hit
CCCAGTGTTTAGACTATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA610080.11562322430280095RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG600340.11377728572964778RNA PCR Primer, Index 1 (100% over 29bp)
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAAT593550.11249043532803485RNA PCR Primer, Index 48 (100% over 41bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG584220.11072220053465506RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCAG578070.10955664383805425RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA577710.10948841612898495RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG575770.10912074458566695RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCCA573400.10867157883429396RNA PCR Primer, Index 1 (100% over 28bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA555300.10524124123942001RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC554200.10503276768393044RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT543980.10309585883201819RNA PCR Primer, Index 1 (100% over 30bp)
CATATGTGATGAATACGTGTTTGGAACTCTGAGGTGGAATTCTCGGGTGCC539600.1022657550383415No Hit

[FAIL]Adapter Content

Adapter graph