FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213462
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATAACTAAATCTTTAG12027156.343049348361774No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGAGTATTGAGTCTGGGACAG2235310.47165303426371No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA185778.702719922047015No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG108225.069754804133757No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA62872.9452548931425735No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG17840.8357459407294975No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATAACTAAATCTTGAG6680.3129362603179957No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATACCTAAATCTTTAG5890.2759273313282926No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATCACTAAATCTTTAG4780.22392744376048193No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATAACTAAAGCTTTAG4550.2131526922824671No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATAACTCAATCTTTAG3840.17989150293729095No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATAACTAACTCTTTAG3830.17942303548172508No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATAACTACATCTTTAG3740.17520682838163235No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA3350.15693659761456372No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATAACTAAAGCTTGAG3200.1499095857810758No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATAACTAAATCTTTCG3080.14428797631428544No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTAACTGAGAAGAGCGAAGCCGC2660.12461234318051925No Hit
TCCGTAGGTGAACCTGCGGAAGGAGCATTACTGAATAACTAAATCTTTAG2620.1227384733582558No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGAATAACCAAATCTTTAG2430.11383759170250443No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGAGTATTTAGTCTGGGACAG2350.11008985205797754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)