FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213462
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCAAGAGCCTAGAGATCCGTTGTTGAAAGTTT11312352.99444397597699No Hit
GCTGCGTTCTTCATCGATGGGAGAACCTAGAGATCCGTTGTCGAAAGTTG2336010.943399762018533No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG187618.788917933871133No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA108645.089430437267524No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG64473.020209686033111No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCTAGAGATCCGTTGTTGAAAGTTT60052.813147070673No Hit
GCTGCGTTCTTCATCGATGCATGAGCCTAGAGATCCGTTGTTGAAAGTTT11650.545764585734229No Hit
GCTGCGTTCGTCATCGATGCAAGAGCCTAGAGATCCGTTGTTGAAAGTTT6980.32699028398497154No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGAGATCCGTTGTTGAAAGTTT6610.30965698812903464No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGAGATCCGTTGTCGAAAGTTT6420.3007561064732833No Hit
GCTGCGTTCTTCATCGATGCAAGAACCTAGAGATCCGTTGTTGAAAGTTT4650.21783736683812574No Hit
GCTGCGTTCTTCATCGATGCCAGAACCTAGAGATCCGTTGTTGAAAGTTT4600.21549502956029645No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGAGATCCGTTGTTGAACGTTT4420.20706261536011092No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGAGATCCGTTGTTGAAAGTTT4140.1939455266042668No Hit
GCTGCGTTCGTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3980.18645004731521303No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG3920.18363924258181782No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGTTGAAAGTTT3700.1733329585593689No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGAGATCCGTTGCTGAAAGTTT3650.17099062128153958No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGAGATCCGTTGTTGAAAGTTT3490.16349514199248577No Hit
GCTGCGTTCTTCATCGATGCGAGAACCTAGAGATCCGTTGTCGAAAGTTG3280.15365732542560268No Hit
GCTGAGTTCTTCATCGATGCAAGAGCCTAGAGATCCGTTGTTGAAAGTTT2930.13726096448079753No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG2870.13445015974740235No Hit
GCTGCGTTCGTCATCGATGGGAGAACCTAGAGATCCGTTGTCGAAAGTTG2740.12836008282504616No Hit
GCTGCGTTCGTCATCGATGCAAGAGCCGAGAGATCCGTTGTTGAAAGTTT2680.12554927809165098No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA2220.10399977513562134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)