FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260368
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG16681864.07008541756284No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT216048.297486634302219No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT137045.263319609168562No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT69932.6858139249062862No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA54492.0928071037915568No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG49121.8865605604375346No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG24090.9252289067780988No Hit
GCTGCGTTCGTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG18010.6917132673754071No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCAAGAGATCCATTGTTAAAAGTTG16480.6329502857494009No Hit
GCTGCGTTCTTCATCGATGGGTGAACCAAGAGATCCGTTGTCGAAAGTTG11140.427855957721379No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT8590.3299176550113685No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT5270.20240582560068823No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG5080.19510846186935413No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG5030.19318810299268727No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGCCGAAAGTTG4950.19011552879002028No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGATATCCGTTGTCGAAAGTTG4880.18742702636268666No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG4580.17590487310268543No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG4380.16822343759601793No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT3990.15324463835801636No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG3960.1520924230320162No Hit
GCTGCGTTCTTCATCGATGCGCAGGTTCACCTACGGA3940.15132427948134947No Hit
GCTGAGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3930.1509402077060161No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGATATCCGTTGTCGAAAGTTG3040.11675781970134579No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG3020.11598967615067904No Hit
GCTGCGTTCTTCATCGAAGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2880.11061267129601181No Hit
GCTGAGTTCGTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2870.11022859952067843No Hit
GCTGCGTTCTTCATCGATTGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2870.11022859952067843No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG2830.10869231241934492No Hit
GCTGCGTTCGTCATCGAAGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2800.1075400970933448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)