FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248062
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG8939636.037764752360296No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG5904823.803726487732906No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAGAGATCCGTTGTTGAGAGTTGT3405213.727213357950834No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG113364.569825285614081No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA61262.469543904346494No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAGAGAGCCGTTGTTGAGAGTTGT16090.6486281655392604No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG14630.5897719118607445No Hit
GCTGCGTTCTTCATCACTAGAATAAGTGGAGTCTCAGCAGCTATCTCCAC13720.5530875345679709No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG10780.4345687771605486No Hit
GCTGCGTTCTTCATCGATGCCTGAACCAAGAGATCCGTTGTTGAAAGTTG7220.29105626819101676No Hit
GCTGCGTTCGTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG6470.26082189130136824No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG5750.2317968894873056No Hit
GCTGAGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG5030.20277188767324297No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCT4490.18100313631269602No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTG4400.1773750110859382No Hit
GCTGCGTTCTTCATCGATGCCTGAACCAGAGATCCGTTGTTGAGAGTTGT4400.1773750110859382No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT4340.17495626093476632No Hit
GCTGCGTTCTTCATCGATGCGAGAACCGAGATATCCGTTGCCGAGAGTCG4210.16971563560722722No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTTG3560.1435125089695318No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAGAGATCCGTTGTTGAGAGTTTT3150.12698438293652392No Hit
GCTGCGTTCGTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG3010.12134063258378953No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGCGTCG2730.11005313187832073No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAGAGCTCCGTTGTTGAGAGTTGT2730.11005313187832073No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGCGAGTCG2680.10803750675234416No Hit
GCTGCGTTCTTCATCGTAGGTGGAGCCTAGAGATCATTTGGAGATGGTTT2670.10763438172714886No Hit
GCTGAGTTCGTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2570.10360313147519572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)