FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223155
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3807617.06257982120051No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTATGAGTTG2930913.133920369250074No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT2319810.395465035513432No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGAGAGTCA203879.135802469135802No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG190458.534426743743138No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT172457.727812507001859No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA72713.2582733974143534No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTAAAAGTTT71733.2143577334139946No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG62982.822253590553651No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG42831.9192937644238308No Hit
GCTGCGTTCTTCATCGACCCACGAGCCAAGTGATCCGCCGTTCAGGGTAA34251.5348076449104882No Hit
GCTGCGTTCTTCATCGATGCACTGAGCCGGGACACGATTAACAGGTGGTCCGCAGGTTCACCTACGGA10770.4826241849835316No Hit
GCTGCGTTCTTCATCGATTCGAGACCCAAGGAATCCATTGTCTCGAGTTG10220.45797763886088144No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTATTATAAGTTG9680.4337792117586431No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCTAGATATCCGTTGCCGAGAGTCA8820.39524097600322644No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCCTTGTTATGAGTTG8400.37641997714592995No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG6460.2894848871860366No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG4790.21464901077726245No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG4330.19403553583831865No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTAGGAGTTG3970.1779032511034931No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTATGAGTTG3730.1671483946136094No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT3670.16445968049113843No Hit
GCTGCGTTCGTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3580.16042660930743205No Hit
GCTGCGTTCTTCATCGATGCCTGAGCCAAGAGATCCATTGTTATGAGTTG3080.13802065828684099No Hit
GCTGCGTTCTTCATCGATGCCTGAGCCAAGAGATCCGTTGTTGAAAGTTT2720.12188837355201541No Hit
GCTGCGTTCTTCATCGATGGGTGAACCAAGAGATCCGTTGTCGAAAGTTG2720.12188837355201541No Hit
GCTGCGTTCTTCATCGATGCATGAGCCTAGATATCCGTTGCCGAGAGTCA2550.11427035020501444No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG2460.11023727902130806No Hit
GCTGCGTTCTTCATCGATGCCTGAACCAAGAGATCCGTTGTTAAAAGTTT2300.10306737469471891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)