FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210396
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG11486354.59371851175878No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG5764627.398809863305384No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA40891.943478012889979No Hit
GCTGCGTTCTTCATCGGTATAGGAGCCTAGATATCCATCGTTGAAAATTT27351.2999296564573473No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG14890.7077130743930493No Hit
GCTGCGTTCGTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG8310.3949694861119033No Hit
GCTGCGTTCTTCATCGATGGGTGAACCAAGAGATCCGTTGTCGAAAGTTG7960.37833418886290615No Hit
GCTGAGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG5840.27757181695469496No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG5230.24857887032072853No Hit
GATGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG5210.24762828190650013No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGCCGAAAGTTG4280.20342592064487916No Hit
GCTGCGTTCTTCATCGATGCAGCGTTCACCTACGGA4150.1972470959523945No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT3760.17871062187494058No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTTG3220.1530447346907736No Hit
GCTGAGTTCGTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3120.14829179261963155No Hit
GCTGCGTTCTTCATCGATGGCAGAACCAAGAGATCCGTTGTCGAAAGTTG2880.13688473164889065No Hit
GCTGCGTTCTTCATCGAAGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2710.1288047301279492No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGATATCCGTTGTCGAAAGTTG2510.11929884598566513No Hit
GCTGAGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2470.11739766915720833No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGCGTCG2380.11312002129318047No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCT2300.10931766763626685No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAACGTTG2170.1031388429437822No Hit
GCTGCGTTCGTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2150.1021882545295538No Hit
GATGCGTTCGTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2150.1021882545295538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)