FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359221
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAAAGCAGAC16036044.641042700732974No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC7647421.288844471787563No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATATGAAGGCGGGCT341529.507239276100227No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGCGAAGCTCGGAATGCGTGT118023.285442666213835No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG100392.794658441460828No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA48121.3395653372158085No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG37431.0419769445550233No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG26480.7371506676948174No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14330.3989187714526712No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC11170.3109506404135616No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAAAGCCGAC5970.16619295642515333No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCAACGCAGAC5960.16591457626363715No Hit
TCCGTAGGTGAACCTGCGGAAGGAGCATTGTCGAAACCTGCAAAGCAGAC5320.14809824592660228No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTTCAAAGCAGAC5040.14030360140414952No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTTGAAGAACGCAGC4630.12889001478198656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)