FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147296
Sequences flagged as poor quality0
Sequence length35-301
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG7193348.835677818813814No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2408916.354144036497935No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG107547.300945035846188No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTGAAAATTT51143.4719204866391484No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT39882.707473386921573No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT33042.243102324570932No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA23901.6225830979795786No Hit
GCTGCGTTCTTCATCGTTATAGGAGCCTAGATATCCATCGTTGAAAATTT9320.632739517705844No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCAAGATATCCGTTGCCGAGAGTCG8920.6055833152291984No Hit
GCTGCGTTCGTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG4930.33470019552465785No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA4490.3048283728003476No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTTAAAATTT4270.2898924614381925No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGATATCCGTTGCCGAGAGTCG3130.21249728437975232No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTTG3040.20638713882250706No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG3010.20435042363675862No Hit
GCTGCGTTCTTCATCGGTATGGGAACCTAGACATCCATCGTTGAAAATTT2980.2023137084510102No Hit
GCTGAGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG2850.19348794264610036No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGATATCCGTTGCCGAGAGTCG2500.1697262654790354No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCT2500.1697262654790354No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGGAG2060.13985444275472517No Hit
GCTGCGTTCGTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1980.13442320225939605No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTAG1700.11541386052574408No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCTGAGAGTCG1620.10998262003041495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)