FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192767
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTAAAAGTTG5438328.211778987067287No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG3929920.386788195074885No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACATTATGGAATCTAAACTAAAG191999.959692270979993No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG46962.4361016149029657No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG46242.3987508235330735No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG39272.0371744126328677No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG16800.8715184652974834No Hit
GCTGCGTTCTTGCTGCGGTTATTGGGTCAGGGCTGGGGCAAGGATCTAAG14680.7615411351528011No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA14270.7402719345116124No Hit
GCTGCGTTCTTCATCAACGCAGAAGGCCAGTGATCCACCGCTAAAAGTCA14210.7371593685641215No Hit
GCTGCGTTCTTCATCGATGCAGAGCGTTCACCTACGGA10140.5260236451259811No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCCCCGCTAAGAGTCG10070.5223923181872415No Hit
GCTGCGTTCTTCATCGATGCATGAGCCGAGTGATCCACCGCTAAGAGTCG9860.511498337371023No Hit
GCTGCGTTCGTCATCGATGCGAGAACCAAGAGATCCGTTGTTAAAAGTTG9760.506310727458538No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACATTATGGAATCTAAACTAACG9210.4777788729398704No Hit
GCTGCGTTCTTCATTGACGCAGAAGCCCAGTGATCCACCGCTAAAAGTCA8090.4196776419200382No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC8070.41864011993754124No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG8070.41864011993754124No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACATTATGGCATCTAAACTAAAG8050.4176025979550442No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACAGTATGGAATCTAAACGAAAG7290.37817676262015804No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6080.3154066826790893No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG5980.3102190727666042No Hit
GCTGCGTTCTTCATCGACGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG5700.2956937650116462No Hit
GCTGCGTTCTTCATCGATGCAGAAGCCCAGTGATCCACCGCTAAAAGTCA5560.28843111113416714No Hit
GCTGCGTTCTTCATCGATGCCACAAAAACATTATGGAATCTAAACTAAAG4910.25471164670301455No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTACGAGTCG4760.246930231834287No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCCAAGAGTCG4460.23136740209683196No Hit
GCTGCGTTCTTCATCTCCGTTATAAGCTTGCAGCTTGTTTTATTCCTTAG4390.2277360751580924No Hit
GCTGCGTTCTTCATCGAAAGTACTGACTTAGCAAGTGCTTCCTGTGCCAC3910.20283554757816433No Hit
GCTGCGTTCTTCATCGATGCAACAACAACATTATGGAATCTAAACTAAAG3790.1966104156831823No Hit
GCTGCGTTCTTCATCGATGCGTGAACCAAGAGATCCGTTGTTAAAAGTTG3750.19453537171818827No Hit
GCTGCGTTCGTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG3480.18052882495447872No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG3270.1696348441382602No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGCGTGATCCACCGCTAAGAGTCG2830.14680936052332608No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGCGATCCACCGCTAAGAGTCG2750.14265927259333808No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTTATCCACCGCTAAGAGTCG2600.13487785772461056No Hit
GCTGAGTTCGTCATCGATGCGAGAACCAAGAGATCCGTTGTTAAAAGTTG2510.13020900880337402No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC2450.127096442855883No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTAAAAGTTT2430.126058920873386No Hit
GCTGCGTTCTTCATCGATGCAGCGTTCACCTACGGA2350.12190883294339798No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGGGATCCACCGCTAAGAGTCG2300.11931502798715547No Hit
GCTGCGTTCTTCATCGATGCAGAAGGCCAGTGATCCACCGCTAAAAGTCA2210.11464617906591897No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACATTATGGACTCTAAACTAAAG2180.11308989609217346No Hit
GCTGCGTTCTTCATCGATGCAACAAACACATTATGGAATCTAAACTAAAG2140.11101485212717944No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTG2060.10686476419719143No Hit
GCTGCGTTCTTCATCGATGCAACAAAAACATTATGGAATCTAAACTACAG2010.10427095924094892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)