FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233188
Sequences flagged as poor quality0
Sequence length35-301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGATAGGGCCTCCGGGC16642571.3694529735664No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG68892.954268658764602No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC41111.7629552121035386No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG31431.3478395114671424No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGATATGGCCTCCGGGC21370.9164279465495652No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG16110.690858877815325No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15010.6436866391066436No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC13450.576787827847059No Hit
TCCGTAGGTGAACCTGCGCATCATTGCAGCCCATGAAGTGGGCCATGCTC12240.5248983652675095No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGATCGGGCCTCCGGGC9020.3868123574111875No Hit
TCCGTAGGTGAACCTGCGGCATCGATGAAGAACGCAGC8260.3542206288488258No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGATAGGGCCGCCGGGC7840.33620941043278385No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGAGAGGGCCTCCGGGC6090.2611626670326089No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGATAGGGCCTCCTGGC5840.25044170368972674No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC5640.24186493301542106No Hit
TCCGTAGGTGAACCTGCGGATGTAGAACATCGATGAAGAACGCAGC5640.24186493301542106No Hit
TCCGTAGGTGAACCTGCGGATGTAGGCATCGATGAAGAACGCAGC5400.23157280820625417No Hit
TCCGTAGGTGAACCTGCGCTAAGAAACGTCCCGTGAAGGTTCGCCCGCCG5220.22385371459937906No Hit
TCCGTAGGTGAACCTGCGGATGTAGAAATGAAGAACGCAGC5020.21527694392507332No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGATAGTGCCTCCGGGC4960.21270391272278164No Hit
TCCGTAGGTGAACCTTCGGAAGGATCATTACCGAGATAGGGCCTCCGGGC4860.20841552738562877No Hit
TCCGTAGGTGAACCTGCATCGATGACGAACGCAGC4730.20284062644733003No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGAGAGGGCCGCCGGGC4670.20026759524503834No Hit
TCCGTAGGTGAACCTGCGGAAGGAGCATTACCGAGATAGGGCCTCCGGGC4490.1925485016381632No Hit
TCCGTAGGTGAACCTGCGGATGTAGAATCGATGAAGAACGCAGC4220.18096986122785047No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA3850.16510283548038493No Hit
TCCGTAGGTGAACCTGCGGGGCAGAGGCGGCGGCCGCAGCGGCGCAGCTG3650.1565260648060792No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGATAGGGACTCCGGGC3300.14151671612604422No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGATAGGGCCCCCGGGC3230.13851484639003722No Hit
TCCGTAGGTGAACCTGCGGCAGGATCATTACCGAGATAGGGCCTCCGGGC2740.11750175823798824No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTAGGGCCTCCGGGC2500.1072096334288214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)