FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219891
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATAACAGGACGTTCAA9052441.16766943622067No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCCGGCTCCGG2297310.447448963350023No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA202219.19591979662651No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTTAGGACGTTCTT160987.320899900405201No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC126875.769676794411777No Hit
TCCGTAGGTGAACCTGCGGTTGGATCATTAATAAATGATTTGGCACTATG113325.153462397278652No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG41151.871381730038974No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATAACCGGACGTTCAA30461.3852317739243534No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACACTAATCCAAACCTACTC24161.0987261870654097No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC13610.6189430217698769No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG13180.599387878539822No Hit
TCCGTAGGTGAACCTGCGGTTGGATCATTACCAATCTTGACTCGAAAGAG12500.5684634659899678No Hit
TCCGTAGGTGAACCTGCCGCATCGATGAAGAACGCAGC6450.29332714845082336No Hit
TCCGTAGGTGAACCTGCGGGTGGATCATTAATAAATGATTTGGCACTATG6380.29014375304127954No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTTCGGACGTTCTT4940.22465676175923527No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC3650.16599133206907057No Hit
TCCGTAGGTGAACCTGCGGAAGGAGCATTAGTGAATAACAGGACGTTCAA3490.158714999704399No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATAACAGGACGTTCAC3070.13961462724713608No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATAACAGGACGCTCAA2840.12915489947292066No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCCGGCCCCGG2730.12415242097220897No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGAATAACAGGACGTTCAA2630.11960471324428922No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATACCAGGACGTTCAA2560.11642131783474538No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAATAACAGGACGTTCCA2410.10959975624286579No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGCGAATAACAGGACGTTCAA2220.10095911155981827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)