FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219891
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT9474743.08816640972118No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3330515.146140587836701No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT2330310.597523318371376No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTT174367.929383194400861No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCTATAGTTT126795.766038628229441No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG19880.9040842963104447No Hit
GCTGCGTTCTTCATCGTTGTAGGAACCTAGATATCCATCGTTGAGGATTT17510.7963036231587468No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCAAAAGTTG15100.686703866915881No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA14940.6794275345512094No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA13250.6025712739493658No Hit
GCTGCGTTCTTCATCGATGCGGCAGGTTCACCTACGGA6510.29605577308757525No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCAAGAGATCCGTTGTTGAAAGTTT5550.2523977788995457No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTT5250.23875465571578644No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT4590.20873978471151614No Hit
GCTGCGTTCGTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT4520.20555638930197234No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG4280.19464189075496496No Hit
GATGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT3630.16508179052348665No Hit
GCTGCGTTCTTCATCGATGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA3580.16280793665952678No Hit
GCTGAGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT3410.1550768335220632No Hit
GCTGCGTTCTTCATCGATGTAGGAACCTAGATATCCATCGTTGAGGATTT3310.15052912579414346No Hit
GCTGCGTTCTTCATCGATGCCTGAACCAAGAGATCCGTTGTTGAAAGTTT3150.14325279342947186No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG2300.10459727774215406No Hit
GCTGCGTTCTTCATCGATGGGTGAACCAAGAGATCCGTTGTCGAAAGTTG2300.10459727774215406No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG2220.10095911155981827No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTGAAAGTTT2200.10004957001423433No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)