FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247621
Sequences flagged as poor quality0
Sequence length35-301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA7680831.018370816691636No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGC7228229.19057753583097No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGTCCTTTGGG2692610.873875802133098No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAATATAATTCGGAGGGG143885.810492648038736No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA124645.033498774336587No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG38251.544699359101207No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTTGGCAGGCCA19830.8008206089144296No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGAGTTGTAGGCTTTGC6330.255632599819886No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAATATACTTCGGAGGGG5480.22130594739541476No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTTTAGGCTTTGC5280.21322908800142154No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTGTACAACTCCCAAA5030.20313301375892998No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA3810.1538641714555712No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTTGGCAGGCCA3720.15022958472827425No Hit
TCCGTAGGTGAACCTGCTGAAGGATCATTACCGAGTGCGGGTCCTTTGGG3710.1498257417585746No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG3390.1369027667281854No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCTGGTCCTTTGGG3320.13407586594028778No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGCGGGGCCTTTGGG2490.10055689945521584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)