FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187800
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTTGC7801741.54259850905218No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA4319022.997870074547393No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCAGAGTGCCCTAGGCTCTC129466.893503727369541No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACAAAACATGAACTACTAAC63343.3727369542066032No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG53052.824813631522897No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG51452.7396166134185305No Hit
TCCGTAGGTGAACCTGCGGTTGGATCATTACCAATCTTGACTCGAAAGAG42012.236954206602769No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAATAATCAATAATCTTGGCT11910.634185303514377No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGAGTTGTAGGCTTTGC6640.35356762513312034No Hit
TCCGTAGGTGAACCTGCGGATCATCGATGAAGAACGCAGC5980.3184238551650692No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC5980.3184238551650692No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTTTAGGCTTTGC5860.3120340788072417No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTGTACAACTCCCAAA3290.1751863684771033No Hit
TCCGTAGGTGAACCTGCGGGTGGATCATTACCAATCTTGACTCGAAAGAG2650.14110756123535678No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCTAGAGTTGTAGGCTTGGC2250.11980830670926518No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTCCAACTCCCAAA2090.11128860489882854No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCTAGTGTTGTAGGCTTTGC2060.10969116080937168No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGAGTTGTAGGCTTGGC1960.10436634717784878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)