FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187800
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG7891942.02289669861555No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT4427523.575612353567625No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT130756.962193823216188No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA53502.8487752928647496No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCAAAAGTTG48802.5985090521831737No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG29951.5947816826411074No Hit
GCTGCGTTCTTCATCGATGTGGGAACCTAGACATCCATCGTTGAAAATTT29821.5878594249201277No Hit
GCTGCGTTCTTCATCGTTGTGGGAACCTAGACATCCATCGTTGAAAATTT29411.5660276890308837No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTAAAAGTTG12510.6661341853035143No Hit
GCTGCGTTCTTCATCGATGCCTGAACCAAGAGATCCGTTGTTGAAAGTTG8690.4627263045793397No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTG8670.46166134185303515No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTG6900.36741214057507987No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA6490.345580404685836No Hit
GCTGCGTTCTTCATCGATGATCCGCAGGTTCACCTACGGA5970.31789137380191695No Hit
GCTGCGTTCTTCATCGATGCCTGAACCAAGAGATCCGTTGTTGAAAGTTT5280.28115015974440893No Hit
GCTGCGTTCGTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG4910.2614483493077742No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT3650.1943556975505857No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG3480.1853035143769968No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG3180.16932907348242812No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT2770.14749733759318423No Hit
GCTGCGTTCGTCATCGATGCCAGAACCAAGAGATCCGTTGTGGAAAGTTG2180.11608093716719914No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAACGTTG2150.11448349307774228No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTGGAAAGTTG2120.11288604898828541No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGATATCCGTTGTTGAAAGTTG2110.11235356762513311No Hit
GCTGCGTTCGTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT2020.10756123535676251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)