FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205726
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTAAAAGTTG3695417.9627271224833No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT3423716.64203843947775No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT2409211.710722028328941No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT2169910.547524377084084No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG193999.429532484955718No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT180488.772833769188143No Hit
GCTGCGTTCTTCATCGTTGTGGGATCCAAGACATCCATTGCTGAAAGTTG91324.438913895180969No Hit
GCTGCGTTCTTCATCGATGTGGGATCCAAGACATCCATTGCTGAAAGTTG39491.9195434704412664No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCCTTGTTGAAAGTTT12510.6080903726315584No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCCTTGTTAAAAGTTG9140.444280256263185No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG6690.32518981557994614No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCGAGATATCCGTTGCCGAGAGTCG5520.26831805411080756No Hit
GCTGCGTTCGTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT5440.2644293866599263No Hit
GCTGCGTTCGTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4380.21290454293574948No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT4350.211446292641669No Hit
GCTGCGTTCTTCATCGATGCCTGAGCCAAGAGATCCATTGTTAAAAGTTG4110.19978029028902522No Hit
GCTGCGTTCGTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT3820.18568387077958062No Hit
GCTGCGTTCTTCATCGGTGTGGGATCCAAGACATCCATTGCTGAAAGTTG3780.18373953705413998No Hit
GCTGCGTTCGTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT3650.1774204524464579No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG3260.1584631986234117No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTAAAAGTTG3180.15457453117253045No Hit
GCTGCGTTCTTCATCGATGCCTGAACCAAGAGATCCGTTGTTGAAAGTTT3140.1526301974470898No Hit
GCTGCGTTCTTCATCGTTGTGGGATCCAAGACATCCCTTGCTGAAAGTTG3000.1458250294080476No Hit
GCTGCGTTCGTCATCGATGCCAGAGCCAAGAGATCCATTGTTAAAAGTTG2990.14533894597668742No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTCGAAAGTTT2860.13901986136900538No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT2690.13075644303588266No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGCTAAAAGTTG2590.1258956087222811No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTT2550.12395127499684046No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTCAAAAGTTG2500.12152085784003966No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCATTGTTAAAAGTTG2330.11325743950691698No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCATTGTTGAAAGTTT2310.11228527264419665No Hit
GCTGCGTTCTTCATCGATGCCTGAGCCAAGAGATCCGTTGTTGAAAGTTT2170.10548010460515443No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGCTGAAAGTTT2120.10304968744835362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)