FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204983
Sequences flagged as poor quality0
Sequence length35-301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCGCACAAACCAGG5975629.151685749549962No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGTTTGGATGCCCCT5849728.537488474653994No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA2311411.276057038876393No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG157497.683076157535015No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGAATCTATCGTGCCCACACTG67663.3007615265656174No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG38531.879668070035076No Hit
TCCGTAGGTGAACCTGCCCATTGTCATCGATGAAGAACGCAGC13500.658591200245874No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC11290.550777381538957No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10670.5205309708609982No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTCACAAACCAGG8970.43759726416336964No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT6920.3375889707926999No Hit
TCCGTAGGTGAACCTGCAGCTGGATCCGATGAAGAACGCAGC6280.3063668694477103No Hit
TCCGTAGGTGAACCTGCTTTGTAAGGTCTCATCCCAGAGTCACGCTTCTC5950.2902679734417No Hit
TCCGTAGGTGAACCTGCTGAGGGATCATTAACTGAGAAGAGCGAAGCCGC3760.1834298454018138No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC3500.17074586673041178No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGTTTGGAGGCCCCT2920.14245083738651498No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTAACGGAGAAGAGCGAAGCCGC2400.11708288004371095No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCGCACACACCAGG2290.11171658137504087No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA2290.11171658137504087No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAGGCCGCACAAACCAGG2120.103423210705278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)