FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204983
Sequences flagged as poor quality0
Sequence length35-301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT5615827.39641823956133No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG5520526.931501636721094No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2331711.375089641580033No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA158817.747471741559056No Hit
GCTGCGTTCTTCATCGTTACGGGAGCCAAGATATCCGTTGTTGAGAGTTG63623.103672011825371No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCGAGATATCCGTTGCCGAGAGTCG35141.7142885019733343No Hit
GCTGCGTTCTTCATCGATGACAATGGGCAGGTTCACCTACGGA13850.6756657869189152No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG12790.6239541815662762No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10550.5146768268588127No Hit
GCTGCGTTCTTCATCGATGCTCACACGTGTACTGAGCAGTGAGAGGGGAA10430.5088226828566271No Hit
GCTGCGTTCTTCATCGATGCATGAGCCGAGATATCCGTTGCCGAGAGTCG8730.42588897615899857No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG6640.32392930145426696No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTCGAAAGTTT6530.31856300278559685No Hit
GCTGCGTTCTTCATCGGATCCAGCTGCAGGTTCACCTACGGA5840.28490167477302997No Hit
GCTGCGTTCTTCTAAAAGTGATGTTGACCCCCGTGTCGCAGGCAGCTGGC5360.26148509876428777No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGCTGAAAGTTT5110.24928896542640122No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT4360.21270056541274154No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4290.20928564807813332No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA3760.1834298454018138No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG3300.16098896006010255No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA3120.15220774405682422No Hit
GCTGCGTTCTTCATCGATACGGGAGCCAAGATATCCGTTGTTGAGAGTTG2860.1395237653854222No Hit
GCTGCGTTCTTCATCGATTCCGGAACCAAGAGATCCGTTGTTGAAAGTTT2680.13074254938214389No Hit
GCTGAGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG2560.12488840537995832No Hit
GCTGCGTTCTTCATCGGTACGGGAGCCAAGATATCCGTTGTTGAGAGTTG2540.1239127147129274No Hit
GCTGCGTTCGTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2480.12098564271183464No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAACGTTT2460.12000995204480372No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGCGATCCGTTGTTGAAAGTTT2290.11171658137504087No Hit
GCTGCGTTCGTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG2270.11074089070800992No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGATATCCGTTGCCGAGAGTCG2180.10635028270637077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)