FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001802727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833385
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC21233025.478020362737507No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG10226912.27151916581172No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGG601937.222712191844105No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA525626.307048962964296No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA461085.53261697774738No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAA405014.859818691241143No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTTGAAACGAGTTG358474.301373314854479No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC342064.1044655231375655No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCTAGCAGAA245222.942457567630807No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC209592.514924074707368No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAATGAATTTAGATTGAACCAT187192.246140739274165No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCACAGCAGAA180332.163825842797747No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG162291.947359263725649No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTGACCCCGGTCTAACC93841.1260101873683832No Hit
TCCGTAGGTGAACCTGCGTAAGGATCATTACTGATTTGCTTAATTGCACC70780.8493073429447374No Hit
TCCGTAGGTGAACCTTCGGAAGGATCATTGTCGATGCCTAAACATCAAAC25780.30934082086910614No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21750.2609838190032218No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGAGTTTTGAAACGCGTTG17680.21214684689549249No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGATCGATGAAGAACGCAGC15930.1911481488147735No Hit
TCCGTAGGTGAACCTGCGGATGTAGAACTCGATGAAGAACGCAGC15920.19102815625431224No Hit
TCCGTAGGTGAACCTGCGGATGTAGGCATCGATGAAGAACGCAGC13340.16007007565530937No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTCAACATCAAAC11990.14387107999304044No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGCTGCCTAAACATCAAAC9160.10991318538250629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)