FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001802728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833385
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG26854632.22352214162722No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA9684511.620679517869892No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG8394010.072175525117443No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT549866.59791092952237No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG445595.346748501592901No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTAAAAGTTG358394.300413374370788No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT271813.2615177858972744No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT253673.0438512812205643No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT191692.3001373914817282No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCGAGAGTCG166752.000875945691367No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG66840.8020302741230044No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA41380.4965292151886583No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG25070.30082134907635727No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGCTATCCGTTGCCGAGAGTCG23920.28702220462331335No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA21940.26326367765198555No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21480.257744019870768No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG21050.2525843397709342No Hit
GCTGCGTTCTTCATCGAGTTCTACATCCGCAGGTTCACCTACGGA19160.22990574584375767No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGCGTCG18930.2271459169531489No Hit
GCTGCGTTCTTCATCGATCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA17450.20938701800488368No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGATAGTCG15950.19138813393569598No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATCTCCGTTGCCGAGAGTCG15600.1871883943195522No Hit
GCTGCGTTCTTCATCGCTGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT14680.17614907875711705No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGCGAGTCG13980.16774959952482946No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG12930.15515038067639805No Hit
GCTGCGTTCTTCATCGATGCCTACATCCGCAGGTTCACCTACGGA12590.15107063362071552No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG10230.12275238935186018No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGCTAAAAGTTG8880.10655339368959124No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG8370.10043377310606741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)