FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224413
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG3526415.713884668000516No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTCGTGGGCGCTCTA2473311.021197524207599No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCACAGCAGAA145826.497841034164688No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAACATCAAAC135526.038865841105462No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA119855.340599697878465No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACACCAAATCAACTGCTA119655.331687558207412No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC96474.298770570332378No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGAATAAGCTTGGTTGGGT83373.7150254218784116No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCCCAGCAGAA80833.6018412480560396No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAAGTAAATATGTGTTCTCC68143.0363659859277314No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGAGTATTTGAGACTGGGACA66132.9467989822336493No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTCACAATCAGAT62472.783706826253381No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAAAACATCAAA59892.668740224496798No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCGAGTTTACAACTCCCAAA50812.2641290834309955No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCTCACGG47202.1032649623684905No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG44321.9749301511053279No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAAACCTGCATAGCAGAA18950.8444252338322646No Hit
TCCGTAGGTGAACCTGCGGAAGGATCACTAGTGATTAAACGGGAAGCGTC18150.8087766751480528No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCAGAGTGCCCTAGGCTCTC14020.6247409909408099No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTTCGATGAAAAACCTTTTTTCA10790.4808099352533053No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG10630.4736802235164629No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC10440.4652136908289627No Hit
TCCGTAGGTGAACCTGCGGCTCGGCATCGATGAAGAACGCAGC8540.3805483639539599No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCACACCAACTCAACTGCTA6590.2936550021611939No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTCAACATCAAAC5710.254441587608561No Hit
TCCGTAGGTGAACCTGCAGATGGATCATTAACACAATTACAAACCTTAAA5700.2539959806250084No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT5680.25310476665790305No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA3060.13635573696710973No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTCTTGGGCGCTCTA3020.13457330903289916No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAAGTAAATCTGTGTTCTCC2950.13145406014803065No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTCAAACATCAAA2440.1087281039868457No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGAGTATTTGAGACTGGGACA2400.1069456760526351No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGAGTATTTGCGACTGGGACA2360.10516324811842451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)