FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224413
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4197518.704353134622327No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT3491115.556585402806434No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT3446915.359627116076163No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG222369.908516886276642No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT95524.2564379068948766No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG85923.8286552026843363No Hit
GCTGCGTTCTTCATCGATGTGGGAACCAAGACATCCGTTGCTGAAAGTGT77923.4721696158422195No Hit
GCTGCGTTCTTCATCGATGGGAGAACCTAGAGATCCGTTGTCGAAAGTTG73593.279221791963924No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA55502.4731187587171863No Hit
GCTGCGTTCTTCATCGTTGTGGGAACCAAGACATCCGTTGCTGAAAGTGT45932.0466728754573045No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG24091.073467223378325No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCGAGAGTCG18630.83016581035858No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT14600.6505861959868635No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGCCAAAAGTTG11940.5320547383618596No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA11380.5071007472829114No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA10000.4456069835526462No Hit
GCTGCGTTCTTCATCGATGCCGAGCCGCAGGTTCACCTACGGA8710.3881236826743549No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG5870.26157129934540335No Hit
GCTGCGTTCTTCATCGTCACGGGAACCTAGATATCCACCGTTGAAAATTT5440.24241019905263955No Hit
GCTGCGTTCTTCATCGATGCCTGAACCAAGAGATCCGTTGTTGAAAGTTT4960.2210210638421125No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG4530.20185996354934876No Hit
GCTGCGTTCTTCATCGATGCCTGAACCAAGAGATCCGTTGTTAAAAGTTT4300.19161100292763789No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTCGAAAGTTT4040.18002522135526908No Hit
GCTGCGTTCTTCATCGATGCGGGAACCAAGACATCCGTTGCTGAAAGTGT3830.1706674747006635No Hit
GCTGCGTTCGTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG3670.16353776296382116No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG3620.16130972804605795No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG3230.14393105568750475No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT3040.13546452300000447No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCGAGATATCCGTTGCCGAGAGTCA3000.13368209506579387No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTAAAAGTTT2540.11318417382237214No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG2520.11229295985526686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)