FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184818
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCTGCTTCATGCAAA6569435.545239100087656No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA2919015.793916176995747No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG2650814.342758822192645No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC1889610.22411237000725No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG39962.162127065545564No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG32691.7687671114285406No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCAGAGTGCCCTAGGCTCTC29431.5923773658409894No Hit
TCCGTAGGTGAACCTGCGCATCATTGCAGCCCATGAAGTGGGCCATGCTC21691.1735869882803622No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCTTCTTCATGCAAA7870.42582432447056023No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA5950.3219383393392419No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCTGCGTCATGCAAA3960.21426484433334417No Hit
TCCGTAGGTGAACGCTGCATCGATGAAGAACGCAGC3870.20939518878031363No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTCAAGGGCCAGC3630.1964094406388988No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAGAGTTCTAAAAGACTCCC3170.17152009003452046No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCTGCTTCATTCAAA2760.14933610362627017No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC2710.14663073943014207No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCTGCTGCATGCAAA2690.14554859375169085No Hit
TCCGTAGGTGAACCTGCGGGTGAATGGAGCACTCAGAGGAGGGAGAGGCA2440.13202177277105043No Hit
TCCGTAGGTGAACCTGAGGAAGGCCCCCCAGGCCCTGTGAGCATCGGTGA2410.1303985542533736No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2320.12552889870034303No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCTGCTTCCTGCAAA2130.11524851475505633No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGGAGCCCGGAGGGCGAGG2050.11091993204125139No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC1870.10118062093519029No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT1860.10063954809596469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)