FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184818
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG6673236.10687270720385No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4964626.86210217619496No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG150538.144769448863206No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA39772.151846681600277No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC30311.6399917756928437No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT29931.6194310078022702No Hit
GCTGCGTTCTTCATCCCCAAGCATGGGTGTAAGGAGAGAGGCCTGGAAGG17930.9701436007315305No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG17560.9501239056801827No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCACCGCTAAGAGTCG15610.8446147020311875No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG11930.6454998971961606No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG8250.44638509236113366No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG6070.32843121340994924No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG6020.32572584921382114No Hit
GCTGCGTTCGTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4610.2494345788830092No Hit
GCTGCGTTCGTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4380.23698990358082006No Hit
GCTGCGTTCTTCATCGATGCAGCGTTCACCTACGGA4170.2256273739570821No Hit
GCTGCGTTCTTCATCGATGCCCGAGCCGAGTGATCCCCCGCTAAGAGTCG4000.21642913569024663No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT3780.20452553322728306No Hit
GCTGCGTTCTTCATCGATGCATGAGCCGAGTGATCCACCGCTAAGAGTCG3720.20127909619192938No Hit
GCTGCGTTCTTCATCGATGGGTGAACCAAGAGATCCGTTGTCGAAAGTTG3680.1991148048350269No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA3100.16773258015994114No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGATATCCGTTGCCGAGAGTCG2990.16178077892845935No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG2930.15853434189310564No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTTG2930.15853434189310564No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGCC2850.1542057591793007No Hit
GCTGCGTTCTTCATCGATGCTCACAGGGCCTGGGGGGCCTTCCTCAGGTT2630.14230215671633717No Hit
GCTGCGTTCTTCATCGATGCGAGAACCGAGATATCCGTTGCCGAGAGTCG2530.136891428324081No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCGAAGAGTCG2440.13202177277105043No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCT2400.12985748141414796No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTAG2340.12661104437879428No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2320.12552889870034303No Hit
GCTGCGTTCTTCATCACGTCCTTGCTTTTCAGAGTCCCGAGCCTTGCTCT2240.1212003159865381No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTT2010.10875564068434891No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTACGAGTCG1950.10550920364899521No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA1870.10118062093519029No Hit
GCTGAGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1870.10118062093519029No Hit
GCTGCGTTCTTCATCAACGCAGAAGGCCAGTGATCCACCGCTAAAAGTCA1870.10118062093519029No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGCGAGTCG1850.10009847525673907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)