FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200703
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG8281341.26146594719561No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTTGGGCAGGCCA3073315.312675944056641No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGCTAATCCAACAACCTAA2104210.48414821901018No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC124226.189244804512139No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG66373.3068763297011006No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC46532.3183509962481876No Hit
TCCGTAGGTGAAACCTTCCAACCCCTCTGAGAGTTCTGTCTCAGTGCTGT20251.0089535283478572No Hit
TCCGTAGGTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGG19410.9671006412460202No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC11630.5794631868980533No Hit
TCCGTAGGTGAACCTGCGCATCGATGAAGAACGCAGC9210.4588870121522847No Hit
TCCGTAGGTGAACCTGCATCCCAGGGATTAAGCACACTTGATCTTGGTGG8720.4344728280095464No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATTGT6120.3049281774562413No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6060.3019386855203958No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAGGGATGAAGCACAATTGATCAT5840.2909772150889623No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGAAGATTTGGGCAGGCCA5340.26606478229024977No Hit
TCCGTAGGTGAACCTGCGGATGGAGCCGCCTGTGGAGCTGGAGCTCGTGG5070.252612068578945No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA4970.2476295820192025No Hit
TCCGTAGGTGAACCTTCCAACCCCTCTGAGAGTTCTGTCTCAGTGCTGTC4960.24713133336322826No Hit
TCCGTAGGTGAACCTGCTGCCTTCAAGGGAAGGATCCCATCTTGGAAGGA4440.22122240325256723No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTATGAAGAACGCAGC4280.21325042475697922No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC3590.17887126749475593No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTT3290.16392380781552843No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG3180.15844307259981166No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGCTTTGACTATACTTTTG2880.14349561292058413No Hit
TCCGTAGGTGAACCTGCATGGCTATCATTAGACTCAAGGAGTTGTTTTTT2580.12854815324135663No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG2570.12804990458538237No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACCTTTG2530.12605690996148536No Hit
TCCGTAGGTGAACCTGCGTGCTCCTGTACTGGGTGCATATACATTTAGGA2060.10263922313069561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)