FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200703
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA7717338.45134352750084No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG4228121.066451423247287No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAAAATTT157347.839444353098858No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC43992.191795837630728No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA39511.9685804397542639No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG37131.8499972596323921No Hit
GCTGCGTTCTTCATCGATGCAGGAGTGGAGTGATCCACCGCTAAGAGTTT24121.2017757582098922No Hit
GCTGCGTTCTTCATCCCGGTTTCAACTCCACTATAGGAACCCTGGAGGAC18300.9117950404328784No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA17690.8814018724184491No Hit
GCTGCGTTCTTCATCGATATGGGAACCAAGATATCCATCGTTGAAAATTT13800.6875831452444657No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG11760.5859404194257186No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA11430.5694982137785684No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG11090.5525577594754438No Hit
GCTGCGTTCTTCATCGCTATGGGAACCAAGATATCCATCGTTGAAAATTT9400.46835373661579544No Hit
GCTGCGTTCTTCATCGATGCGCAGGTTCACCTACGGA8580.4274973468259069No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG7230.36023377826938313No Hit
GCTGCGTTCTTCATCGATGCAAACATTCTCAATAAAATACTGGCAAACCG7190.3582407836454861No Hit
GCTGCGTTCTTCATCGATGTGAGAACCAAGAGATCCGTTGTCGAAAGTTG6410.31937738847949454No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5940.2959597016487048No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAGTAGAATACTGGCAAACTG5750.28649297718519406No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA4780.23816285755569175No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG4630.23068912771607797No Hit
GCTGCGTTCTTCATCGGTATGGGAACCAAGATATCCATCGTTGAAAATTT4240.2112574301330822No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA4180.20826793819723668No Hit
GCTGCGTTCTTCATACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA4110.20478019760541694No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA4090.20378370029346843No Hit
GCTGCGTTCTTCATCCTGGGCTCTGTAGTGGCTATTTATTGTTGCACCTG4050.20179070566957147No Hit
GCTGCGTTCTTCATCGATGCCAGTTACGCTTTTTAGCTACACAATTTCTG3910.19481522448593194No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCATCGTTGAAACTTT3680.1833555053985242No Hit
GCTGCGTTCTTCATCGATGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA3550.17687827287085894No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATCGTTA3130.15595182931994042No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGCTA3030.1509693427601979No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGCAAGTTG2920.14548860754448115No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG2750.1370183803929189No Hit
GCTGCGTTCTTCATCGGTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA2590.12904640189733088No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGCTGTTGATAGTTA2550.12705340727343387No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAACGTTG2510.12506041264953688No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG2470.12306741802563988No Hit
GCTGCGTTCTTCATCGTTATGGGAACCAAGATATCCCTCGTTGAAAATTT2460.12256916936966562No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTCA2450.12207092071369137No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG2230.11110945028225787No Hit
GCTGCGTTCTTCATCGATGCTTTGAGGGTTTTCTCAAAACTGATGTTTGA2160.10762170969043812No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGCTCCGTTGTCGAAAGTTG2070.10313747178666986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)