FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300056
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG15011850.029994401045144No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAACATCAAACG4662315.53809955474978No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG255638.519409710187432No Hit
TCCGTAGGTGAACCTGCGACCCTGCCTCGGCACCGCCAGCGGAAGCCGGG84972.8318047297837734No Hit
TCCGTAGGTGAACCTGCGGGGACAAGGGCATGGGGTGGTAGGAGAAAAGG59071.9686325219292398No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC35221.1737808942330765No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT32211.0734662862932252No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTGTCGATGCCTAACATCCAACG27880.929159890153838No Hit
TCCGTAGGTGAACCTGCGGGGCAGAGGCGGCGGCCGCAGCGGCGCAGCTG23340.7778548004372516No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC14330.47757751886314553No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC12030.4009251606366812No Hit
TCCGTAGGTGAACCTGCATGAGGCAGCGGTCCCTCAGAGAGTCTGTTTCT10960.36526515050523906No Hit
TCCGTAGGTGAACCTGCTGAGGCCAGGAGAAGGAGGTCATAGGGCAGCAG9730.3242728024102168No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT9400.3132748553603327No Hit
TCCGTAGGTGAACCTGCGTGCTCCTGTACTGGGTGCATATACATTTAGGA9030.3009438238195537No Hit
TCCGTAGGTGAACCTGCGGTGTTTGCGTGGCATCGATGAAGAACGCAGC8070.2689497960380729No Hit
TCCGTAGGTGAACCTGCGGTGTTTGCATGGCATCGATGAAGAACGCAGC7750.25828512011091265No Hit
TCCGTAGGTGAACCTGCGGGCCTACAGCAGTCGTTACAAAGTGCAGGACA7010.23362305702935454No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC6770.22562455008398433No Hit
TCCGTAGGTGAACCTGCTCACACACCCAGTACGTCATTATTGCAGCCAGCATCGATGAAGAACGCAGC6270.20896099394779644No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA6210.20696136721145386No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGCCTATACTTTTG6140.20462846935238757No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTT6070.20229557149332122No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6040.20129575812514997No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGCTTTGACTATACTTTTG4830.16096995227557523No Hit
TCCGTAGGTGAACCTGCGGTCGTCCTGTTGTTCTTGGAGGCATCGATGAAGAACGCAGC4640.15463780094382382No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATCCTTTTG4450.1483056496120724No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACCTTTG4140.1379742448076359No Hit
TCCGTAGGTGAACCTGCGACCCTGCCTCGGCCCCGCCAGCGGAAGCCGGG3710.1236435865305143No Hit
TCCGTAGGTGAACCTGCGTGCCTGTGGCTCACTCCAGGGGTGTGTTGCGT3420.11397872397152532No Hit
TCCGTAGGTGAACCTGCGGAAGGCTCATTATTGATTTGACTATACTTTTG3220.10731330151705015No Hit
TCCGTAGGTGAACCTGCGGGGACAAGGGCATGGGGTGGTAGGAGACAAGG3130.10431386141253633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)