FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300056
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA13769845.89076705681606No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4775715.916029007918523No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG132764.424507425280614No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA73902.4628735969285733No Hit
GCTGCGTTCTTCATCCACAGTAAACACTGCTAACTACCAACTCATAAAAC71222.3735569360386064No Hit
GCTGCGTTCTTCATCCCCCCATGGAAGAAGTGATCCAAGAGGTATTTTCA50321.6770202895459514No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA33381.112459007651905No Hit
GCTGCGTTCTTCATCGATGCTCACACGTGTACTGAGCAGTGAGAGGGGAA30441.01447729757112No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG30311.0101447729757111No Hit
GCTGCGTTCTTCAAATGCGGGGGCAGGCGTGAAGCTGGCCCGACGCACCC18340.6112192390753726No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC13670.45558162476337755No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG11710.39026048470952085No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA11560.3852614178686645No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGCGTCG11070.36893113285520035No Hit
GCTGCGTTCTTCATCCCTCTGTGTAGCACATGGACTGCCCACACTCAGGG9670.3222731756738742No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA9340.3112752286239902No Hit
GCTGCGTTCTTATCAGACGATGGCAAATTAATGATTGATGATCAACAATA8920.29727784146959235No Hit
GCTGCGTTCTTCATCGATGCCACGCAAACACCGCAGGTTCACCTACGGA8610.28694643666515585No Hit
GCTGCGTTCTTCATCGATGCTAGGAAGAAACTGCATCAACTAACGAGCAA8170.2722825072653105No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA8100.2699496094062442No Hit
GCTGCGTTCTTCATCGATGCCATGCAAACACCGCAGGTTCACCTACGGA7950.2649505425653878No Hit
GCTGCGTTCTTCATCGATGCCACTGTGCTTTCTCCCAGCCAGGCCCTACT7730.2576185778654651No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCACCGCTAAGAGTCG7590.25295278214733247No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCCCCGCTAAGAGTCG7320.243954461833791No Hit
GCTGCGTTCTTCATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG7120.23728903937931586No Hit
GCTGCGTTCTTCATCGATGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA6990.232956514783907No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA6610.2202922121204042No Hit
GCTGCGTTCTTCATCGATGCAAATGGGCTGGATGTACGCATTGTAGGTGA6390.2129602474204815No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATCGTTA6340.21129389180686273No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGCTA6130.2042951982296638No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGCGATCCACCGCTAAGAGTCG6090.20296211373876877No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5960.1986295891433599No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG5950.19829631802063616No Hit
GCTGCGTTCTTCATCGATGCTGGCTGCAATAATGACGTACTGGGTGTGTGAGCAGGTTCACCTACGGA5550.18496547311168582No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGGGATCCCCCGCTAAGAGTCG5360.1786333217799344No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGCTGTTGATAGTTA5060.16863518809822167No Hit
GCTGCGTTCTTCATCGGTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA4960.1653024768709841No Hit
GCTGCGTTCTTCATCGCTGCACGAGCCGAGTGATCCACCGCTAAGAGTCG4840.16130322339829897No Hit
GCTGCGTTCTTCATCGATGCCTCCAAGAACAACAGGACGACCGCAGGTTCACCTACGGA4610.15363798757565256No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGCTAGTTA4480.1493054629802437No Hit
GCTGCGTTCTTCATCCACAGTAAACACTGCTAACTACCAACTCATAACAC4440.14797237848934866No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTCA4360.14530620950755857No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTACGAGTCG4290.14297331164849228No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGCGATCCCCCGCTAAGAGTCG4210.14030714266670222No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGCTCCACCGCTAAGAGTCG4150.13830751593035967No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGCTATCCGTTGCCGAGAGTCG3690.12297704428506678No Hit
GCTGCGTTCTTCATCGATGCCAAAGGGTTCAGGATTTCCTCCGCCCTCCC3420.11397872397152532No Hit
GCTGCGTTCTTCATCCACAGTAAACACTGCTAACTACCAACTCATACAAC3270.10897965713066893No Hit
GCTGCGTTCTTCATCGATGCGGGGGCAGGCGTGAAGCTGGCCCGACGCAC3230.1076465726397739No Hit
GCTGCGTTCTTCATCGATGCTCACACGTGTACGGAGCAGTGAGAGGGGAA3150.10498040365798385No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGATAGTCG3070.10231423467619379No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGCTCCGTTGTTGATAGTTA3050.10164769243074627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)