FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001802737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336317
Sequences flagged as poor quality0
Sequence length35-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTG7876023.41838206216159No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAAAATGAACTTTTCT4758114.147664257233503No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAGTGATAAAACTATTATCTTA3448310.253124284529179No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACC328579.769651846323558No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGG238867.1022279575519525No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGAACGCCCGGGCTTCGG204126.069273929060976No Hit
TCCGTAGGTGAACCTGCAGAAGGATCATTAGTGAAGATTCAAGGGCCAGC153424.561767618050827No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGTTAGGGTCCTAGTGG103343.0726962954593438No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATTGT32250.9589167362934374No Hit
TCCGTAGGTGAACCTGCTCCAGAAACTGAAGTTTGAAATGGAGCTCCTAA29740.8842847670501343No Hit
TCCGTAGGTGAACCTGCCTTGCATCCCAAGTATGAAGCCGACTTGATCGT24290.7222352720796154No Hit
TCCGTAGGTGAACCTGCTGAGGCCAGGAGAAGGAGGTCATAGGGCAGCAG15590.4635507571725485No Hit
TCCGTAGGTGAACCTGCTTACCTCAGAGCCTGCTGCGGACTGAGTTGTCT14870.44214238352506713No Hit
TCCGTAGGTGAACCTGCATCGATGAAGAACGCAGC14350.4266807803352195No Hit
TCCGTAGGTGAACCTGCGGAGCATCGATGAAGAACGCAGC14010.4165712705572421No Hit
TCCGTAGGTGAACCTGCGGCTTCCTGGGAGAGAGGAGAAAAGTCAAAGGC13490.40110966736739445No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAACGGAGCCCGGAGGGCGAGG13090.389216126452127No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGCAAGG12760.37940395519703135No Hit
TCCGTAGGTGAACCTGCCCATTGTGCATCGATGAAGAACGCAGC8370.2488723436519712No Hit
TCCGTAGGTGAACCTGCGGAGGGATCATTACCAGAACGCCCGGGCTTCGG7100.21111035124599709No Hit
TCCGTAGGTGAACCTGCCCATTGTGGAGCGGCCGGTGCATCGATGAAGAACGCAGC5960.17721375963748487No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTTACAACAAAATGAACTCTTCT5500.16353618758492733No Hit
TCCGTAGGTGAACCTGCGAGGTGCTTCTAGTGTTACCTTTACAGCATCGATGAAGAACGCAGC5420.16115747940187383No Hit
TCCGTAGGTGAACCTGCCACAGCGGATTAGCGAGGCGGAACAGAGGGGAC5350.15907610974170203No Hit
TCCGTAGGTGAACCTGCGGAATCTGATGATAAAGCTTTAACATCGTAAAT4680.13915442870862907No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTTCATGCCCGAAAGG4570.13588370495693053No Hit
TCCGTAGGTGAACCTGCTGCCAGCCAGGCCTGCAGCGCCCGGGGCGATTT4230.1257741951789532No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAGTTAGGGTCCTCGTGG3500.10406848300859012No Hit
TCCGTAGGTGAACCTGCGGAAGGATCATTATTGATTTGACTATACTTTTT3410.10139243630265495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)