FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336317
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA7199721.407481631912752No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT4244612.620830942236044No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTGAAAATTT3837411.41006847706182No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG327479.736944608806573No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT247347.354371024955622No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGGTCCGTTGTTAAAAGTTT195485.812373445291199No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG147294.379499103524354No Hit
GCTGCGTTCTTCATCGATGCAAAAATCCTCAATAAAATACTGGCAAACCG51281.524751945337286No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGATAGTTA41801.2428750256454477No Hit
GCTGCGTTCTTCATCGATGCTCCCTGCCCTGACCACCAGGCCATGCATGG27650.8221410157678619No Hit
GCTGCGTTCTTCATCGATATGGGAACCTAGACATCCATCGTTGAAAATTT21560.641061855332915No Hit
GCTGCGTTCTTCATCGGTATGGGAACCTAGACATCCATCGTTGAAAATTT17800.5292625707294011No Hit
GCTGCGTTCTTCATCGCTGTGAGAGCCAAGAGATCCGTTGTTGATAGTTA16690.49625799468953397No Hit
GCTGCGTTCTTCATCGATGCCATCCTGAGGCTCCACTCTGAGCTCATCTA14840.4412503679564221No Hit
GCTGCGTTCTTCATCGATGCTCCGCAGGTTCACCTACGGA14210.42251804101487583No Hit
GCTGCGTTCTTCATCGATGCAGGTTCACCTACGGA14160.4210313484004674No Hit
GCTGCGTTCTTCATCGCTATGGGAACCTAGACATCCATCGTTGAAAATTT14060.41805796317165056No Hit
GCTGCGTTCTTCATCGATGCCACTGTGCTTTCTCCCAGCCAGGCCCTACT12490.37137581507922585No Hit
GCTGCGTTCTTCATCGATGCTCACACGTGTACTGAGCAGTGAGAGGGGAA11320.3365872079020686No Hit
GCTGCGTTCTTCATCGCTGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT10210.3035826318622014No Hit
GCTGCGTTCTTCATCGATGCACAATGGGCAGGTTCACCTACGGA7960.23668146421382208No Hit
GCTGCGTTCTTCATCGCTGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG7180.21348905942905055No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCATCGTTGAAACTTT6660.1980274562392029No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG6560.19505407101038605No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT6110.18167383748071017No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG6010.17870045225189332No Hit
GCTGCGTTCTTCATCGATGCACCGGCCGCTCCACAATGGGCAGGTTCACCTACGGA6000.1784031137290116No Hit
GCTGCGTTCTTCATCGATGCTGTAAAGGTAACACTAGAAGCACCTCGCAGGTTCACCTACGGA5510.163833526107809No Hit
GCTGCGTTCTTCATCGTTATGGGAACCTAGACATCCCTCGTTGAAAATTT5280.15699474008153022No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGCTGATAGTTA5200.15461603189847675No Hit
GCTGCGTTCTTCATCGCTGCCAGAACCAAGAGGTCCGTTGTTAAAAGTTT5070.15075063110101483No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGCGATCCGTTGTTGATAGTTA5020.1492639384866064No Hit
GCTGCGTTCTTCATCGATGCCGGGTGAGAGGAGAACGCGGCCTGGGGCGC5010.1489665999637247No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTCGAAAGTTG4690.13945176723151076No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGCTGAAAGTTT4540.13499168938828546No Hit
GCTGCGTTCTTCATCGATATGGGAACCTAGACATCCCTCGTTGAAAATTT3640.10823122232893372No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT3540.10525783710011685No Hit
GCTGCGTTCTTCATCGCTGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3480.10347380596282672No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCAAGAGATCCGTTGTCGATAGTTA3420.10168977482553662No Hit
GCTGCGTTCTTCATCGGTATGGGAACCTAGACATCCCTCGTTGAAAATTT3410.10139243630265495No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGCGATCCGTTGTTGAAAGTTT3400.10109509777977325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)