FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204746
Sequences flagged as poor quality0
Sequence length37-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT201859.858556455315366No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT148947.2743789866468695No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT108785.312924306213553No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT101854.974456155431608No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT63093.0813788791966634No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT54042.639367802057183No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT40161.9614546804333173No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT34961.7074814648393621No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT32891.6063805886317684No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT27811.358268293397673No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT26171.2781690484795796No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC25721.2561905971301028No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT25401.2405614761704746No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT24811.2117452844011605No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA24311.1873247829017417No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT24011.1726724820020904No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT23431.1443447002627645No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC22881.117482148613404No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT21921.07059478573452No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT21021.026637883035566No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC19270.9411661277876002No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA18450.9011165053285534No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC17880.873277133619216No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT17490.8542291424496693No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT16990.8298086409502506No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16680.8146679300206109No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT16630.812225879870669No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT16020.7824328680413781No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15230.7438484756722964No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC15020.7335918650425405No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC14160.6915886024635401No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC13940.6808435818037959No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC12870.6285837085950396No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA12660.6183270979652838No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA12600.6153966377853535No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC12580.6144198177253768No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT12560.6134429976654001No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT12530.6119777675754349No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC11760.57437019526633No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11560.5646019946665625No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11520.562648354546609No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11340.5538569740068182No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11190.5465308235569926No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTAGACAATGGGCGCAAGCCTGAT11110.5426235433170856No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10610.5182030418176667No Hit
CCTACGGGGGGCTGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC10240.5001318707080968No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT9300.45422132788918956No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTAGACAATGGGCGCAAGCCTGAT8820.43077764644974753No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7990.3902396139607123No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7830.3824250534808983No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC7670.3746104930010843No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT7400.3614234221913981No Hit
CCTACGGGTGGCTGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC7380.3604466021314214No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7020.3428638410518398No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6930.3384681507819445No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC6900.33700292069197935No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6690.32674631006222343No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6380.3116055991325838No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT6170.3013489885028279No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTAGACAATGGGCGCAAGCCTGAT6140.29988375841286274No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT6030.2945112480829906No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6010.2935344280230139No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA5910.2886503277231301No Hit
CCTACGGGCGGCTGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC5890.28767350766315336No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTAGACAATGGGCGCAAGCCTGAT5820.28425463745323476No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC5170.2525079855039903No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT5070.24762388520410658No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT5030.24567024508415305No Hit
CCTACGGGAGGCTGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC4910.23980932472429253No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT4750.23199476424447854No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4670.22808748400457152No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4650.22711066394459478No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT4550.222226563644711No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC4180.20415539253514112No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4120.20122493235521086No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4060.1982944721752806No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4060.1982944721752806No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4030.1968292420853155No Hit
CCTACGGGGGGCAGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC3970.19389878190538523No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3690.1802233010657107No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT3680.1797348910357223No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC3680.1797348910357223No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT3610.1763160208258037No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTAGACAATGGGCGCAAGCCTGAT3560.1738739706758618No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3510.1714319205259199No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT3450.16850146034598967No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3440.1680130503160013No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT3420.16703623025602454No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3310.16166371992615242No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3300.16117530989616405No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3270.15971007980619892No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3230.1577564396862454No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3200.15629120959628026No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC3200.15629120959628026No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3190.1558027995662919No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3050.14896505914645464No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3010.1470114190265011No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2980.14554618893653598No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA2940.1435925488165825No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCACAAGCCTGAT2780.1357779883367685No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT2740.133824348216815No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2610.1274750178269661No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC2590.12649819776698934No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2560.1250329676770242No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC2520.12307932755707071No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2480.1211256874371172No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT2430.11868363728717533No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2400.1172184071972102No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2400.1172184071972102No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2350.11477635704726832No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT2320.1133111269573032No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCACAAGCCTGAT2270.11086907680736133No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGAA2190.1069617965674543No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGGCGCAAGCCTGAT2170.10598497650747755No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2170.10598497650747755No Hit
CCTACGGGTGGCAGCAGACGAGAATATTCCGCAATGGACGAAAGTCTGAC2140.10451974641751242No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2130.10403133638752404No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2120.10354292635753569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)