FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206408
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG78773.8162280531762334No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG76093.6863881244912986No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG72673.520696872214255No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG48922.3700631758458974No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG47162.2847951629781793No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG45442.201465059493818No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG45392.1990426727646213No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG44592.1602844850974767No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG44272.144781210030619No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG43462.105538545017635No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG40401.9572884771908063No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG39321.9049649238401614No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG39011.8899461261191426No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG37581.8206658656641215No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG37011.793050656951281No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG31491.5256191620479826No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG29841.4456803999844967No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG29151.4122514631215843No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG28911.4006240068214408No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG28481.3797914809503506No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27621.3381264292081703No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG27211.3182628580287585No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG26851.3008216735785434No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG26101.2644858726405952No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG23961.1608077206309833No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG23161.1220495329638387No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG22461.088136118755087No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17580.8517111739855044No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG16840.8158598503933957No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG16830.8153753730475564No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG16520.8003565753265378No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG12560.6085035463741715No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11170.5411611953025076No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG10690.5179062827022208No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10500.5087012131312739No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG10000.48447734583930857No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG9990.48399286849346923No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9960.4825394364559513No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTCTCGCGCCTCAG9290.4500794542847177No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG9280.44959497693887834No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8990.43554513390953836No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG8960.43409170187202045No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG8950.43360722452618117No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG8740.4234332002635557No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG8490.41132126661757296No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG8330.40356962908414407No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG8080.39145769543816133No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG7970.3861284446339289No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG7630.3696562148753924No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG7610.36868726018371384No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG7520.36432696407116005No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7430.3599666679586062No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6910.3347738459749622No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA6830.3308980272082478No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6820.33041354986240845No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG6820.33041354986240845No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6470.31345684275803265No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6420.3110344560288361No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6250.30279834114956783No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG6210.3008604317662106No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG6170.2989225223828534No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6150.29795356769117476No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6010.29117088484942444No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG6000.2906864075035851No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG5820.2819658152784776No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG5800.28099686058679896No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG5790.2805123832409597No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG5680.27518313243672726No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCTCCTCAG5660.27421417774504864No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTCTCGCGCCTCAG5620.2722762683616914No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG5620.2722762683616914No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG5360.2596798573698694No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG5110.24756792372388667No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4970.24078524088213632No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTCTCGCGCCTCAG4920.2383628541529398No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4900.2373938994612612No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG4890.2369094221154219No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG4870.23594046742374328No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG4760.2306112166195109No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTCTCGCGCCTCAG4740.22964226192783224No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4530.2194682376652068No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4420.21413898686097438No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4370.21171660013177784No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTCTCGCGCCTCAG4330.20977869074842062No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG4300.20832525871090266No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4190.20299600790667027No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4130.20008914383163445No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4120.1996046664857951No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4080.1976667571024379No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4040.19572884771908067No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4020.19475989302740204No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG3980.1928219836440448No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3940.19088407426068757No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTCTCGCGCCTCAG3860.1870082554939731No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3720.18022557265222278No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTCTCGCGCCTCAG3680.17828766326886555No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3640.1763497538855083No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG3450.16714468431456145No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3330.16133095616448975No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG3310.16036200147281113No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTCTCGCGCCTCAG3300.15987752412697182No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3270.1584240920894539No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTCTCGCGCCTCAG3250.15745513739777528No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG3110.15067245455602496No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG3100.15018797721018565No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG3050.1477655904809891No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG3040.1472811131351498No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2990.14485872640595326No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2950.14292081702259601No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2930.14195186233091742No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCAG2900.14049843029339948No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2810.1361381341808457No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2750.13323127010580985No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG2640.12790201930157746No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2630.12741754195573815No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2600.12596410991822024No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2510.12160381380566644No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2490.12063485911398783No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2460.1191814270764699No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2350.11385217627223751No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2270.10997635750552304No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2210.10706949343048719No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2200.10658501608464789No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAAG2180.10561606139296927No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2080.10077128793457618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)