FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192670
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG97705.0708465251466235No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG58443.0331655161675406No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG53652.784553900451549No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG53372.770021279908652No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG52532.7264234182799605No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG48142.4985726890538227No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG37561.9494472413972077No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG36101.8736700057092437No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG35601.8477188975969272No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG34661.7989308143457727No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG31851.6530855867545542No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28441.4760990294285565No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG27371.4205636580681995No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG27341.4190065915814605No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCATGCTTTCGCACCCCAG26161.3577619764363937No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG24571.2752374526392278No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG21721.1273161363990243No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG20451.0614003217937407No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG20181.0473867234130898No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19991.0375253023304096No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG19220.9975605958374422No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG19190.9960035293507032No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG18710.9710904655628796No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG18320.9508486012352728No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG18140.9415062023148388No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG16620.862614833653397No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15430.800851196346084No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG14680.7619245341776094No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG13560.7037940520060206No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG13470.6991228525458036No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCATGCTTTCGCACCCCAG13310.6908184979498625No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG12430.6451445476721855No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCATGCTTTCGCACCCCAG12410.6441065033476929No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12380.642549436860954No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11890.6171173509108838No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCATGCTTTCGCACCCCAG11530.5984325530700161No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG11400.5916852649608139No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCATGCTTTCGCACCCCAG11360.5896091763118285No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG11230.5828618882026263No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11200.5813048217158873No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG11130.5776716665801629No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG10970.5693673119842217No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10670.5537966471168319No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9690.5029324752166917No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9260.48061452224009965No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9150.47490527845539005No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9120.47334821196865107No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTGAG8970.4655628795349562No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG8560.4442829708828567No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG8470.43961177142263974No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG8280.4297503503399595No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG8120.4214459957440183No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCATGCTTTCGCACCCCAG8020.41625577412155496No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7760.40276119790315046No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7720.4006851092541652No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7690.39912804276742614No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG7630.3960139097939482No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCATGCTTTCGCACCCCAG7620.3954948876317019No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7570.3928997768204702No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7520.3903046660092386No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7470.3877095551980069No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7460.3871905330357606No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCATGCTTTCGCACCCCAG7410.38459542222452897No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG7360.3820003114132974No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6750.35033995951627134No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6440.3342502724866352No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGCTCCTCAG6410.33269320599989616No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6380.33113613951315723No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6270.3254268957284476No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6260.3249078735662013No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6100.31660351897026No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5990.3108942751855504No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5900.30622307572533347No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCATGCTTTCGCACCCCAG5890.3057040535630871No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG5850.3036279649141019No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG5740.2979187211293922No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG5690.29532361031816057No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5580.28961436653345096No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5420.2813100119375097No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACACACGCTTTCGTGCATGAG5350.27767685680178544No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG5320.27611979031504646No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG5250.27248663517932215No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTGAG4880.25328281517620804No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTGAG4870.2527637930139617No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG4820.2501686822027301No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG4740.24601650490475943No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTGAG4520.2345980173353402No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTGAG4510.2340789951730939No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4500.23355997301084758No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4200.21798930814345774No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4120.2138371308454871No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4020.20864690922302384No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3990.20708984273628486No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3940.20449473192505319No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGCTCCTCAG3840.19930451030258992No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGCTCCTCAG3650.18944308921990968No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3550.1842528675974464No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3540.18373384543520008No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGCTCCTCAG3470.18010069029947579No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACACACGCTTTCGTGCATGAG3460.17958166813722945No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTGAG3460.17958166813722945No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3450.17906264597498314No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3410.1769865573259978No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3390.17594851300150516No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGCTCCTCAG3380.17542949083925882No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACACACGCTTTCGTGCATGAG3360.17439144651476618No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTGAG3360.17439144651476618No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACACACGCTTTCGTGCATGAG3210.16660611408107126No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2960.15363056002491307No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG2940.15259251570042043No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACACACGCTTTCGTGCATGAG2840.14740229407795713No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGCTCCTCAG2770.14376913894223284No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTGAG2690.1396169616442622No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2670.13857891731976954No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG2550.1323506513728136No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2540.1318316292105673No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTGAG2510.1302745627238283No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTCGCTTTCGTCTCTCAG2490.12923651839933564No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2480.1287174962370893No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGCTCCTCAG2420.12560336326361135No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACACACGCTTTCGTGCATGAG2260.1172990086676701No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGCTCCTCAG2250.11677998650542379No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGCTCCTCAG2110.10951367623397519No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG2110.10951367623397519No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACACACGCTTTCGTGCATGAG2070.10743758758498988No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACACACGCTTTCGTGCATGAG2010.10432345461151192No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1950.10120932163803394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)