FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205687
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG103455.029486549952112No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG73813.58846208073432No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG60932.9622679119244286No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG60352.9340697273041076No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG52102.5329748598598845No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG51022.4804678953944586No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG41892.0365895754228514No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG41282.006932864011824No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG39831.936437402461021No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG39511.920879783360154No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG37741.8348267027084844No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG37581.8270478931580507No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG36301.7648174167545834No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG35211.7118242766922556No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG34031.6544555562578092No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG33691.6379255859631383No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG27841.3535128617754162No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27641.3437893498373743No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27221.3233699747674865No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG26471.2869068049998298No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG23241.129872087200455No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG23211.1284135604097487No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG23011.118690048471707No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG20931.0175655243160724No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19560.9509594675404862No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG19190.932970970455109No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG18420.8955354494936482No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG17870.8687957916640333No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG16390.796841803322524No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG15710.7637818627331819No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15120.7350975025159587No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG14870.7229431125934065No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG14780.7185675322212877No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG14610.7103025470739521No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG14440.7020375619266167No Hit
GACTACTGGGGTATCTAATCCGGTTTGCTCCCCTAGCTTTCGGGCCTCAG14150.6879384696164561No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13940.6777287820815122No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG13790.6704361481279808No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12680.616470656871849No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12610.6130674276935344No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG12390.6023715645616884No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11180.5435443173365356No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10780.5240972934604521No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10620.5163184839100187No Hit
GACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG10430.5070811475688789No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG10410.5061087963750748No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG10040.4881202992896974No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10010.48666177249899123No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG9900.48131384093306817No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG9840.4783967873516557No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9420.45797741228176797No Hit
GACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG9260.45019860273133444No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG9040.43950273959948855No Hit
GACTACAGGGGTATCTAATCCGGTTTGCTCCCCTAGCTTTCGGGCCTCAG9000.43755803721188014No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG8890.43221010564595724No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG8880.4317239300490551No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG8780.42686217408003424No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG7870.38262019476194414No Hit
GACTACTCGGGTATCTAATCCGGTTTGCTCCCCTAGCTTTCGGGCCTCAG7870.38262019476194414No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG7740.37629991200221696No Hit
GACTACTAGGGTATCTAATCCGGTTTGCTCCCCTAGCTTTCGGGCCTCAG7620.3704658048393919No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7280.35393583454472083No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG7260.3529634833509167No Hit
GACTACCGGGGTATCTAATCCGGTTTGCTCCCCTAGCTTTCGGGCCTCAG7070.343726147009777No Hit
GACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG7040.3422676202190707No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6880.33448881066863734No Hit
GACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG6870.3340026350717352No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6500.3160141379863579No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6280.3053182748545119No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6140.2985118164978827No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5970.2902468313505472No Hit
GACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG5970.2902468313505472No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG5750.27955096821870123No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGAG5720.27809244142799494No Hit
GACTACACGGGTATCTAATCCGGTTTGCTCCCCTAGCTTTCGGGCCTCAG5720.27809244142799494No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG5710.2776062658310929No Hit
GACTACCAGGGTATCTAATCCGGTTTGCTCCCCTAGCTTTCGGGCCTCAG5310.25815924195500933No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG5240.2547560127766947No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG5230.2542698371797926No Hit
GACTACCCGGGTATCTAATCCGGTTTGCTCCCCTAGCTTTCGGGCCTCAG5220.2537836615828905No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5100.24794955442006544No Hit
GACTACAAGGGTATCTAATCCGGTTTGCTCCCCTAGCTTTCGGGCCTCAG5050.245518676435555No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5000.24308779845104456No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4930.23968456927272994No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG4740.23044723293159022No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG4590.2231545989780589No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4560.22169607218735263No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4240.20613845308648576No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4210.2046799262957795No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG4210.2046799262957795No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4170.20273522390817114No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG4160.20224904831126905No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3990.19398406316393355No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3770.18328820003208757No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3230.15703471779937478No Hit
GACTACTGGGGTATCTAATCCGGTTTGCTCCCCTCGCTTTCGGGCCTCAG3230.15703471779937478No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG3140.15265913742725595No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3120.15168678623345178No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG3100.15071443503964763No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3050.14828355705513718No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2810.13661534272948705No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2730.13272593795427032No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2670.12980888437285779No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2610.12689183079144525No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2410.11716831885340347No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCAG2340.11376508967508885No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2310.1123065628843826No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2200.1069586313184596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)