FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256223
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG88643.4594864629639024No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG64082.5009464411859983No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG60932.378006658262529No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG53922.1044168556296663No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG52022.030262700850431No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG46921.8312173380219576No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG45491.775406579424954No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG41641.625146844740714No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG37371.4584951390000118No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG35431.3827798441201609No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG35311.3780964238183144No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG34291.3382873512526199No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG34251.3367262111520042No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG34141.3324330758753118No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG32671.275061177177693No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG32571.2711583269261542No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG31521.2301783992849977No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG31081.2130058581782277No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG29991.1704647904364558No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG28571.1150443168646063No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG25000.9757125628846748No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG24580.959320591828212No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG24250.9464411859981344No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG23950.9347326352435184No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG23010.8980458428790545No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG22840.8914109974514387No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG22400.8742384563446686No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG21680.8461379345335899No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG21600.8430156543323588No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG19840.7743254899052778No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19580.7641780792512772No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG19160.7477861081948147No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG18010.7029033303021196No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG17690.6904142094971958No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16570.6467022866799623No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG16380.6392868712020389No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG16350.6381160161265773No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG16100.6283588904977304No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG15550.6068932141142677No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG14980.5846469676804971No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG14860.5799635473786506No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14500.5659132864731113No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG14490.5655230014479574No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG13900.5424961849638792No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG12260.4784894408386445No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG12190.47575744566256734No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG11400.44492492867541167No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG11140.434777518021411No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10900.4254106774177181No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG10870.42423982234225655No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG10490.4094089913864095No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10360.40433528605940916No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10020.3910655952041776No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9910.38677245992748505No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9900.3863821749023312No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG9710.3789667594244076No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9540.37233191399679183No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG9540.37233191399679183No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG9530.371941628971638No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG9450.36881934877040706No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9300.36296507339309897No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9200.3590622231415603No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG9150.35711079801579093No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG9020.35203709268879063No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG8860.3457925322863287No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG8840.34501196223602093No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8820.3442313921857132No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8700.33954797188386676No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8700.33954797188386676No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8650.3375965467580974No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8560.33408398153171265No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG8540.33330341148140485No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8440.32940056122986616No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8230.3212045757016349No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8150.31808229550040396No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8100.31613087037463455No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG8090.31574058534948074No Hit
GACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG7970.3110571650476343No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7940.3098863099721727No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7780.30364174956971074No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7650.29856804424271044No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG7640.29817775921755657No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG7550.29466519399117175No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG7530.293884623940864No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7420.28959148866417145No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG7350.28685949348809436No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7330.28607892343778657No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7260.28334692826170954No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7160.2794440780101708No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7130.2782732229347092No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG7070.275931512783786No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6730.2626618219285544No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6600.2575881166015541No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG6570.2564172615260925No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG6560.25602697650093864No Hit
GACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG6560.25602697650093864No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG6490.25329498132486156No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG6450.2517338412242461No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6400.24978241609847673No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG6330.24705042092239962No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG6290.24548928082178414No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG6290.24548928082178414No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6250.2439281407211687No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG6250.2439281407211687No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6230.24314757067086093No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6170.2408058605199377No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6150.24002529046962995No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6050.23612244021809126No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG6050.23612244021809126No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG6050.23612244021809126No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5970.2330001600168603No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG5900.23026816484078322No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG5890.22987787981562938No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5830.22753616966470613No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5750.22441388946347518No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5750.22441388946347518No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5690.22207217931255194No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGCACCTCAG5540.2162179039352439No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5370.20958305850762812No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG5150.20099678795424297No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5140.20060650292908913No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG5070.19787450775301205No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5070.19787450775301205No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5070.19787450775301205No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5020.1959230826272427No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4940.19280080242601172No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4910.1916299473505501No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4870.19006880724993463No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4770.18616595699839592No Hit
GACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG4570.17836025649531853No Hit
GACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG4550.1775796864450108No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4490.17523797629408758No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG4440.17328655116831823No Hit
GACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG4390.17133512604254889No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG4310.1682128458413179No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4280.16704199076585632No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4250.1658711356903947No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4240.16548085066524082No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCGCACTTTCGAGCTTCAG4100.16001686031308665No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4060.15845572021247117No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCAG4030.15728486513700957No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG3780.1475277395081628No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3610.14089289408054703No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG3480.1358191887535467No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3480.1358191887535467No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3460.13503861870323897No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3420.1334774786026235No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGCACCTCAG3390.13230662352716188No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3110.12137864282285352No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGCACCTCAG2910.11357294231977613No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG2900.11318265729462226No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2860.11162151719400679No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCCGCTTTCGCGCCTCAG2770.10810895196762195No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGCACCTCAG2750.1073283819173142No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG2710.10576724181669873No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCGCACTTTCGAGCTTCAG2620.10225467659031391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)