FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185748
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG96095.173137799599457No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG94915.109610870641945No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG56953.0659818679070567No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG55582.9922260266597758No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG54112.913086547365247No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG51252.759114499213989No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG49292.653595193487951No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG47812.5739173503886987No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG46992.529771518401275No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG46922.526002971768202No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG35681.9208820552576606No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG35171.893425501216702No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG34551.860046945323772No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG33861.8228998427977692No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG33191.7868294678812155No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG32311.7394534530654437No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG28201.5181859293235997No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27861.4998815599629605No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG26681.4363546310054482No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19911.0718823352068394No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG16200.8721493636539828No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15540.8366173525421539No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13220.711716949846028No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12570.6767233025389237No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11830.6368843809892973No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11420.6148114649955855No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10730.5776643624695824No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10620.571742360617611No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10040.5405172599435795No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9860.5308267114585352No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG9860.5308267114585352No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9530.5130607059026208No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG8530.4592243254301527No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCCCAG7970.42907595236557056No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7820.42100049529470035No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7600.40915649159075734No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7570.40754140017658336No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7480.40269612593406123No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG6980.3757779356978272No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG6920.37254775286947905No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGACTCAG6870.3698559338458557No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6490.3493981092663178No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6230.3354006503434761No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6110.32894028468677994No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6100.3284019208820552No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6010.32355664663953315No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCTTTCGTTCCTCAG5580.30040700303637186No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG5580.30040700303637186No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG5460.2939466373796757No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG5230.281564269871008No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4720.2541077158300493No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4550.24495553114972976No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4400.23688007407885953No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGACTCAG4310.2320347998363374No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4270.22988134461743867No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4250.22880461700798932No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCCCAG4130.22234425135129313No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4120.22180588754656844No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG4080.21965243232766976No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCCCAG4040.21749897710877103No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG3950.2126537028662489No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3900.20996188384262549No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3890.2094235200379008No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG3840.2067317010142774No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG3720.20027133535758124No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3680.1981178801386825No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCCCAG3650.19650278872450846No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3630.1954260611150591No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGACTCAG3570.19219587828671103No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTCCCCTGGCTTTCGTTCCTCAG3550.19111915067726168No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCCCAG3520.1895040592630876No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3480.1873506040441889No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3380.1819669659969421No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGACTCAG3380.1819669659969421No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG3350.18035187458276805No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3300.17766005555914466No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3290.17712169175441997No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3290.17712169175441997No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG3270.17604496414497062No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3270.17604496414497062No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3260.17550660034024593No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG3240.17442987273079658No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3240.17442987273079658No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG3210.17281478131662253No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3210.17281478131662253No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG3190.17173805370717315No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3160.1701229622929991No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGACTCAG3160.1701229622929991No Hit
GACTACAGGGGTATCTAATCCCTTTCGCTCCCCTGGCTTTCGTTCCTCAG3140.16904623468354976No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3060.1647393242457523No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3050.1642009604410276No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3040.16366259663630295No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2970.15989405000323018No Hit
GACTACCGGGGTATCTAATCCCTTTCGCTCCCCTGGCTTTCGTTCCTCAG2920.15720223097960678No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCTTTCGTTCCTCAG2870.15451041195598336No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2850.153433684346534No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2780.14966513771346124No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2750.14805004629928722No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2750.14805004629928722No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCCCAG2710.14589659108038847No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGACTCAG2700.1453582272756638No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2600.139974589228417No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2600.139974589228417No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2600.139974589228417No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCCCAG2590.1394362254236923No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGACTCAG2590.1394362254236923No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2560.1378211340095183No Hit
GACTACCAGGGTATCTAATCCCTTTCGCTCCCCTGGCTTTCGTTCCTCAG2550.1372827702047936No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2550.1372827702047936No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCTTTCGTTCCTCAG2510.13512931498589487No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTCTCGCTCCTCAG2470.13297585976699614No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2450.13189913215754678No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCCCAG2440.1313607683528221No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2440.1313607683528221No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2410.12974567693864805No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2370.12759222171974935No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGACTCAG2340.1259771303055753No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2300.12382367508667656No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCAAGCTTTCGTGCCTCAG2270.12220858367250254No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2260.12167021986777785No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2220.11951676464887913No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG2170.11682494562525574No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2110.11359476279690765No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGACTCAG2110.11359476279690765No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2100.11305639899218296No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2080.1119796713827336No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2060.11090294377328422No Hit
GACTACCCGGGTATCTAATCCCTTTCGCTCCCCTGGCTTTCGTTCCTCAG2040.10982621616383488No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCACCCCAG2040.10982621616383488No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2040.10982621616383488No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2020.10874948855438551No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2000.10767276094493614No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG2000.10767276094493614No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1930.10390421431186339No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1930.10390421431186339No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1920.1033658505071387No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG1910.10282748670241403No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1860.10013566767879062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)