FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194306
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG104415.373483062797855No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG96844.983891387810979No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG57582.9633670602040083No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG56362.9005794983170876No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG53822.769857853077105No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG53772.767284592344035No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG52452.699350508990973No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG52162.684425596739164No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG49472.5459841692999703No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG47232.430702088458411No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG42532.1888155795497823No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG36461.8764217265550214No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG35871.8460572499047894No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG34341.7673154714728314No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG33601.7292312126233877No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG33461.7220260825707903No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG32941.6952641709468572No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG28841.4842567908350746No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28061.4441139233991744No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG24461.2588391506180974No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG24081.2392823690467614No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG23281.1981101973176331No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19871.0226138153222237No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG19681.0128354245365556No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG15100.7771247413872963No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG15050.7745514806542257No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14950.7694049591880848No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14460.7441870040039937No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG14040.7225716138462014No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12600.6484617047337705No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG10770.5542803619033895No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG10470.5388407975049664No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG9820.5053884079750497No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG9680.4981832779224522No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG9650.4966393214826099No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA9610.4945807128961535No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG9040.4652455405391496No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8720.44877667184749825No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG8680.44671806326104185No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8490.4369396724753739No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG8360.43024919456939054No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8300.42716128168970596No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8030.4132656737311251No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG7760.39937006577254436No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG7560.3890770228402623No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7130.36694698053585584No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTATCGCGCCTCAG6860.35305137257727504No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6720.34584624252467755No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6580.3386411124720801No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6540.3365825038856237No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG6080.3129085051413749No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6030.31033524440830446No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6000.3087912879684621No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5730.2948956800098813No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5610.28871985425051205No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG5580.28717589781066977No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5500.2830586806377569No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG5430.2794561156114582No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5420.2789414634648441No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG5310.27328028985208896No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5240.2696777248257903No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5230.2691630726791761No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG5190.2671044640927197No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG5140.26453120335964925No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5090.2619579426265787No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG4840.2490916389612261No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4830.24857698681461202No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4800.2470330303747697No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG4650.23931324817555813No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG4620.23776929173571584No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4520.2326227702695748No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4470.23004950953650427No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4320.2223297273372927No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4300.2213004230440645No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4280.2202711187508363No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG4160.21409529299146707No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG4150.21358064084485298No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4110.21152203225839653No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4030.2074048150854837No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4020.20689016293886964No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3910.20122898932611447No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTATCGCGCCTCAG3900.20071433717950038No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTATCGCGCCTCAG3780.19453851142013115No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3740.19247990283367472No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTATCGCGCCTCAG3540.18218685990139266No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3480.17909894702170803No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3400.17498172984879518No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG3290.16932055623604006No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3280.16880590408942595No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG3270.16829125194281186No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3160.16263007833005672No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3160.16263007833005672No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3160.16263007833005672No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG3150.16211542618344263No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3090.15902751330375797No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3020.15542494827745926No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2950.15182238325116054No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTCTCGCGCCTCAG2950.15182238325116054No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2880.14821981822486183No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTATCGCGCCTCAG2860.14719051393163363No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2670.13741212314596565No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2640.13586816670612334No Hit
GACTACTGGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG2640.13586816670612334No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTATCGCGCCTCAG2580.1327802538264387No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATCAG2560.1317509495332105No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2470.12711908021368357No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2400.12351651518738485No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTATCGCGCCTCAG2390.12300186304077074No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2380.12248721089415664No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2380.12248721089415664No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG2370.12197255874754254No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTATCGCGCCTCAG2350.12094325445431434No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG2340.12042860230770022No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2320.11939929801447202No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG2210.11373812440171686No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2180.11219416796187456No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2170.11167951581526046No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2160.11116486366864635No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTGTCGCGCCTCAG2080.10704764649573353No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2000.1029304293228207No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1950.10035716858975019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)