FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167530
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG84225.027159314749597No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG55953.33970035217573No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG49012.9254461887423147No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG47492.834716170238166No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG44102.6323643526532563No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG41722.490300244732287No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG33912.0241150838655764No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG31901.9041365725541695No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG31221.8635468274338924No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG30621.8277323464454125No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG30471.8187787261982928No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG29991.790127141407509No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28261.6868620545573927No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG25511.522712350026861No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG24191.4439204918522055No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG21881.3060347400465588No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG21131.2612666388109592No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20291.2111263654270878No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG16791.0022085596609562No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG16360.9765415149525457No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG15270.9114785411568077No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA14810.8840207723989734No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG14780.8822300483495493No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG13550.8088103623231659No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13130.7837402256312301No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13110.7825464095982809No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12490.745538112576852No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12250.73121232018146No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10280.6136214409359517No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9740.5813884080463201No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9700.5790007759804214No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9450.5640780755685548No Hit
GACTACAGGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG9020.5384110308601445No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9000.5372172148271952No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG8470.505581089954038No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8360.49901510177281677No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8350.49841819375634216No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG8350.49841819375634216No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8170.48767384945979825No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8030.479317137229153No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG7940.473944965080881No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7890.4709604249985077No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7750.4626037127678625No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG7700.4596191726854892No Hit
GACTACCGGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG7650.4566346326031158No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG7480.4464871963230466No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7410.442308840207724No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG7340.43813048409240135No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG7280.43454903599355343No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7120.42499850772995884No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA7100.4238046916970095No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7050.42082015161463615No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG6950.4148510714498896No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG6940.4142541634334149No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6910.4124634393839909No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6800.4058974512027696No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6440.3844087626096818No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6110.364710798066018No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6090.3635169820330687No Hit
GACTACCCGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG5970.35635408583537276No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5870.35038500567062614No Hit
GACTACCAGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG5810.3468035575717782No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5790.3456097415388289No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5220.31158598459977316No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG5210.31098907658329855No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCACGCTTTCGAGCATGAG5130.30621381245150125No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG5050.30143854831970396No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5000.29845400823733065No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4920.29367874410553335No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4810.28711275592431207No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG4780.2853220318748881No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4760.28412821584193876No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA4740.28293439980898943No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG4660.27815913567719214No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG4640.2769653196442428No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4590.2739807795618695No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4560.2721900555124455No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG4560.2721900555124455No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4550.27159314749597085No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4530.27039933146302153No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG4270.25487972303468037No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4270.25487972303468037No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG4130.2465230108040351No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4110.2453291947710858No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4080.2435384707216618No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4030.24055393063928848No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG4020.23995702262281385No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3940.23518175849101655No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3930.2345848504745419No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3920.23398794245806723No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3910.23339103444159257No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3890.2321972184086432No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG3750.22384050617799797No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3690.22025905807915No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3550.21190234584850476No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG3540.2113054378320301No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA3500.20891780576613142No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG3480.2077239897331821No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3110.18563839312361965No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3050.18205694502477168No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG3040.18146003700829702No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG2940.1754909568435504No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2900.17310332477765178No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG2820.16832806064585448No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2780.16594042857995583No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG2670.15937444039873455No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2600.15519608428341194No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2580.1540022682504626No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2570.15340536023398793No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2540.15161463618456394No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2500.14922700411866532No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2470.14743628006924134No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2440.14564555601981735No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2440.14564555601981735No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG2340.13967647585507073No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2310.13788575180564674No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA2250.1343043037067988No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG2230.13311048767384945No Hit
GACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2200.13131976362442546No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAG2200.13131976362442546No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG2190.1307228556079508No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG2140.1277383155255775No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2060.12296305139378022No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2030.12117232734435623No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGAA2030.12117232734435623No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1900.11341252313018563No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1890.11281561511371097No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1840.10983107503133767No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1820.10863725899838836No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1810.1080403509819137No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1680.10028054676774309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)