FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166641
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG85765.146392544451846No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG48862.9320515359365342No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG48842.9308513511080707No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG45312.7190187288842482No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG40742.4447764955803195No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG40242.41477187486873No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG35802.148330842949814No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG32041.9226960951986605No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG31081.8650872234324087No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG29681.7810742854399577No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG24761.4858288176379162No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG22321.3394062685653592No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG21951.3172028492387828No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG21481.2889985057698885No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG21031.261994347129458No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTGAG20951.2571936078156036No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19851.1911834422501064No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19391.163579191195444No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19271.1563780822246625No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG18131.0879675470022383No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17361.0417604311063904No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG14570.8743346475357205No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG14140.8485306737237535No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG14050.8431298419956674No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG14000.8401293799245084No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG13670.8203263302548592No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTGAG13080.7849208778151836No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13060.78372069298672No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG13060.78372069298672No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG12380.7429144088189581No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12170.7303124681200905No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTGAG11960.7177105274212229No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG11410.6847054446384744No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11100.6661025797972887No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10940.6565011011695802No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10910.6547008239268848No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG10510.6306971273576131No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTGAG10460.6276966652864541No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10300.6180951866587454No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9860.5916911204325466No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTGAG9070.544283819708235No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG8990.5394830803943808No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG7890.4734729148288836No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG7860.47167263758618827No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7790.4674719906865657No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTGAG7640.45847060447308885No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7540.45246968033077095No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTACCCTAGCTTTCGCGTCTGAG7500.45006931067384376No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTAGTCTCTGAG7450.44706884860268487No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG7410.4446684789457577No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTGAG7310.4386675548034397No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7310.4386675548034397No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG7260.43566709273228077No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTGAG7190.43146644583265825No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6910.414663858234168No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6850.4110633037487773No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6720.40326210236376403No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6680.4008617327068369No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6680.4008617327068369No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6540.3924604389075918No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG6510.39066016166489637No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTGAG6300.37805822096602876No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6180.3708571119952473No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG6080.36485618785292934No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5960.35765507888214787No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG5820.3492537850829028No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5820.3492537850829028No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5740.34445304576904845No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG5730.34385295335481664No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5710.34265276852635307No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5620.3372519367982669No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG5280.316848794714386No Hit
GACTACAGGGGTATCTAATCCCGTTTGCTACCCTAGCTTTCGCGTCTGAG5180.3108478705720681No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5090.305447038843982No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4940.2964456526305051No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4760.28564398917433287No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4710.28264352710317386No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4630.2778427877893196No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG4580.27484232571816064No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4510.270641678818538No Hit
GACTACTCGGGTATCTAATCCCGTTTGCTACCCTAGCTTTCGCGTCTGAG4480.2688414015758427No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4390.26344056984775654No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4370.262240385019293No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4230.2538390912200479No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG4070.24423761259233923No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4060.24363752017810741No Hit
GACTACCGGGGTATCTAATCCCGTTTGCTACCCTAGCTTTCGCGTCTGAG4000.24003696569271665No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTAGTCTCTGAG3980.2388367808642531No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3950.2370365036215577No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3880.23283585672193519No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3880.23283585672193519No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTAGTCTCTGAG3860.2316356718934716No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3820.22923530223654442No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3790.22743502499384904No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3770.22623484016538548No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTAGTCTCTGAG3750.22503465533692188No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTAGTCTCTGAG3710.22263428567999471No Hit
GACTACTAGGGTATCTAATCCCGTTTGCTACCCTAGCTTTCGCGTCTGAG3600.216033269123445No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3600.216033269123445No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3600.216033269123445No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3590.21543317670921322No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3570.21423299188074962No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3480.2088321601526635No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3470.20823206773843173No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGATTTCGTGCTTCAG3330.19983077393918663No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3100.18602864841185543No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG3100.18602864841185543No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2830.16982615322759703No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2800.16802587598490168No Hit
GACTACCCGGGTATCTAATCCCGTTTGCTACCCTAGCTTTCGCGTCTGAG2790.1674257835706699No Hit
GACTACCAGGGTATCTAATCCCGTTTGCTACCCTAGCTTTCGCGTCTGAG2680.16082476701412018No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2670.16022467459988837No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2670.16022467459988837No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTAGTCTCTGAG2660.1596245821856566No Hit
GACTACACGGGTATCTAATCCCGTTTGCTACCCTAGCTTTCGCGTCTGAG2590.15542393528603404No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTAGTCTCTGAG2540.1524234732148751No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTACCCTAGCTTTCGCGTCTGAG2460.14762273390102076No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTAGTCTCTGAG2440.14642254907255717No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTAGTCTCTGAG2400.14402217941563003No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2390.14342208700139822No Hit
GACTACCGGGGTATCTAATCCTATTTGCTCCCCACGATTTCGTGCTTCAG2360.14162180975870284No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2320.13922144010177567No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGATTTCGTGCTTCAG2290.13742116285908032No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2240.13442070078792134No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2120.12721959181713982No Hit
GACTACAGGGGTATCTAATCCTATTTGCTCCCCACGATTTCGTGCTTCAG2010.12061857526059012No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1950.11701802077519938No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1920.115217743532504No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1880.11281737387557685No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1670.10021543317670922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)