FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205914
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC165678.04559184902435No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT122345.941315306390046No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC104135.056965529298639No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC87134.2313781481589405No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT85764.164845518031799No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC75623.6724069271637676No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT64353.125091057431743No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT61042.9643443379274843No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT53352.5908874578707617No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC40541.9687830842001999No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT38741.881367949726585No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT36211.758501121827559No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT34401.6706003477179792No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA29701.4423497188146508No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT28261.3724176112357587No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT25351.2310964771700805No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT24681.1985586215604573No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC21451.0416970191439145No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19090.9270860650562855No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA18270.8872636149071942No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT17310.8406422098545995No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT16710.8115038316967277No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT16080.7809085346309624No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15150.7357440484862613No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT14560.7070913099643541No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC14330.6959215983371698No Hit
CCTACGGGGGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT14330.6959215983371698No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGCAAGTCTGAC14290.6939790397933118No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA13890.6745534543547306No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCGCAATGGACGAAAGTCTGAC13360.6488145536486106No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA13190.6405586798372136No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT13030.632788445661781No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12940.6284176889381003No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC12300.5973367522363705No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT12270.5958798333284769No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT11540.5604281399030664No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT9040.43901823091193404No Hit
CCTACGGGTGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT9030.43853259127596955No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT8890.4317336363724662No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA8830.428819798556679No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC8320.404052177122488No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCGCAATGGACGAAAGTCTGAC8310.4035665374865235No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT8290.40259525821459446No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGTGCGAGCCTGAT7890.38316967277601327No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT7820.3797701953242616No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT7700.37394251969268727No Hit
CCTACGGGCGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT7540.3661722855172548No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA7350.3569451324339287No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7340.3564594927979642No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCGCAATGGACGAAAGTCTGAC6840.33217751099973775No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCGCAATGGACGAAAGTCTGAC6650.3229503579164117No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT6550.31809396155676645No Hit
CCTACGGGAGGCTGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT6470.3142088444690502No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT6150.29866837611818525No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT6050.29381197975853995No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5960.2894412230348592No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5940.2884699437629301No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC5760.27972843031556865No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT5700.27681459249978146No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5370.260788484512952No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC5250.2549608088813777No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC5070.24621929543401613No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGTGCGAGCCTGAT4940.23990598016647727No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTTGGCAATGGACGCAAGTCTGAC4940.23990598016647727No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA4870.23650650271472556No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT4810.23359266489893837No Hit
CCTACGGGGGGCAGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT4790.23262138562700932No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT4450.21610963800421534No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4250.2063968452849248No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC4050.19668405256563418No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA3840.1864856202103791No Hit
CCTACGGGGGGCAGCAGTCGAGAATCTTCCGCAATGGACGAAAGTCTGAC3760.18260050312266285No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGTGCGAGCCTGAT3750.18211486348669834No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGACGAAAGTCTGAT3740.1816292238507338No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3560.17288771040337228No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3560.17288771040337228No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGTGCGAGCCTGAT3500.16997387258758512No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT3500.16997387258758512No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3480.16900259331565604No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3480.16900259331565604No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3280.15928980059636547No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTTGGCAATGGACGCAAGTCTGAC3250.1578328816884719No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3240.15734724205250736No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGTGCAAGCCTGAA2960.14374933224550054No Hit
CCTACGGGGGGCTGCATGGACAGAACTTTGGACCCCTCTCCTGCTCAAAC2950.14326369260953603No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT2910.1413211340656779No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2910.1413211340656779No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2820.13695037734199716No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2680.13015142243849373No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2680.13015142243849373No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2640.12820886389463562No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2570.12480938644288393No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT2570.12480938644288393No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGGTGCGAGCCTGAT2520.12238118826306127No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTTGGCAATGGACGCAAGTCTGAC2500.12140990899113223No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2440.11849607117534505No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2390.11606787299552239No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2320.11266839554377071No Hit
CCTACGGGTGGCAGCAGTGAGGAATCTTCCACAATGGGCGAAAGCCTGAT2160.10489816136833824No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTTGGCAATGGACGCAAGTCTGAC2110.1024699631885156No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2100.10198432355255106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)