FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001802776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001802776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205914
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG115495.608652155754344No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG66403.2246471828044716No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG65933.201822119914139No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG56162.727352195576794No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG54492.646250376370718No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG54082.6263391512961722No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG41662.023174723428227No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG39601.9231329584195342No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG38501.8697125984634362No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG37591.8255193915906642No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG36581.7764697883582468No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG35291.7138222753188226No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG29721.44332099808658No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG27911.3554202239770001No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG26751.2990860262051147No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG24071.168934603766621No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG23461.1393105859727848No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCAG23331.1329972707052458No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG21661.0518954514991696No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21031.0213001544334042No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG20991.0193575958895462No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19630.9533106053983702No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19140.9295142632361083No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG18470.8969764076264849No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG18140.8809502996396554No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG17310.8406422098545995No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG17230.8367570927668831No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG17090.8299581378633798No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG16650.8085899938809405No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG15860.7702244626397429No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG15540.7546839942888779No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG14980.7274881746748643No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCAG13720.6662975805433337No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG13440.6526996707363268No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCAG13340.6478432743766815No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG12500.6070495449556611No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12380.6012218693240867No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12120.588595238789009No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11480.5575143020872791No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11470.5570286624513145No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCAG11070.5376030770127335No Hit
GACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG11040.5361461581048399No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCAG10670.5181774915741524No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10640.5167205726662587No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG10530.5113785366706489No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10340.5021513835873228No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10040.487582194508387No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG9610.46669969016191226No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9430.4579581767145507No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9320.4526161407189409No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG9060.4399895101838631No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9050.43950387054789863No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8160.39628194294705554No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCAG8130.39482502403916203No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCAG7380.3584020513418223No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7350.3569451324339287No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTAGCTTTCGTGCCTCAG7270.3530600153462125No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCAG7150.3472323397146382No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7000.33994774517517024No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6820.33120623172780866No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCCTCAG6670.32392163718834077No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6500.3156657633769438No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG6360.3088668084734404No Hit
GACTACTCGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG6310.3064386102936177No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6230.30255349320590147No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG6120.2972114572102917No Hit
GACTACAGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG5960.2894412230348592No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG5950.28895558339889466No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5850.2840991870392494No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5640.27390075468399433No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCAAACTGTCGTCCCTCAT5620.27292947541206525No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5520.26807307905241995No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG5460.26515924123663276No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG5360.26030284487698746No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5290.2569033674252358No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5180.25156133142962595No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG5080.24670493506998067No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG5000.24281981798226446No Hit
GACTACTAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG4950.24039161980244178No Hit
GACTACCGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG4800.23310702526297386No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTAGCTTTCGTGCCTCAG4630.22485115145157689No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG4560.22145167399982515No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4540.2204803947278961No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4530.21999475509193156No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTAGCTTTCGTGCCTCAG4400.2136814398243927No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTAGCTTTCGTGCCTCAG4280.20785376419281834No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4190.2034830074691376No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4160.202026088561244No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4040.19619841292966966No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCAAACTGTCGTCCCTCAT4020.1952271336577406No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCAAACTGTCGTCCCTCAT3990.19377021474984701No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG3920.1903707372980953No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG3670.1782297463989821No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCCTCAG3610.17531590858319493No Hit
GACTACACGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG3580.17385898967530133No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG3540.17191643113144323No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG3540.17191643113144323No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG3520.17094515185951417No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTAGCTTTCGTGCCTCAG3500.16997387258758512No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3490.16948823295162058No Hit
GACTACCCGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG3480.16900259331565604No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG3470.16851695367969152No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG3470.16851695367969152No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG3450.16754567440776247No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCAAACTGTCGTCCCTCAT3390.1646318365919753No Hit
GACTACAAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG3370.16366055732004622No Hit
GACTACCAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTAG3360.1631749176840817No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG3280.15928980059636547No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3250.1578328816884719No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTAGCTTTCGTGCCTCAG3230.15686160241654282No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAATCTCAG3160.1534621249647911No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG3090.1500626475130394No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTTCTCAG3070.14909136824111038No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTAGCTTTCGTGCCTCAG3000.14569189078935868No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCAAACTGTCGTCCCTCAT2990.14520625115339414No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2900.14083549442971335No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2820.13695037734199716No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTACTCAG2780.13500781879813903No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2760.13403653952620997No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG2740.13306526025428092No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTAGCTTTCGTGCCTCAG2730.13257962061831638No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2640.12820886389463562No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCAAACTGTCGTCCCTCAT2630.1277232242586711No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG2610.12675194498674203No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTAGCTTTCGTGCCTCAG2600.1262663053507775No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCAAACTGTCGTCCCTCAT2520.12238118826306127No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2510.12189554862709676No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2450.1189817108113096No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTGTCGTCCCTCAT2310.11218275590780617No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2280.11072583699991258No Hit
GACTACAGGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCCTCAG2270.11024019736394805No Hit
GACTACTCGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCCTCAG2210.10732635954816087No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2180.1058694406402673No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG2160.10489816136833824No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2140.10392688209640917No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCAAACTGTCGTCCCTCAT2120.10295560282448013No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCAAACTGTCGTCCCTCAT2110.1024699631885156No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCGCCTCAG2080.101013044280622No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTCCATCAG2060.10004176500869295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)