FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527589
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTA1341428.78128868432543TruSeq Adapter, Index 6 (97% over 40bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT66920.43807594843901076No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG58820.38505121469190995No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA31800.20817117693306247No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA29940.195995126961506No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT26410.17288681706925096No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA24160.15815772436172296No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT23920.15658662113958663No Hit
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA20720.13563857817776903No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA19210.12575372040516133No Hit
TGTATCGCCAATCACACCGGTTGAGACCGCACCAGCGACACAAGATCGGA18900.12372437874323525No Hit
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT18670.12221873815535461No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT17700.11586886263255365No Hit
CTGTGTAGCAGAAGACAGGGCATGAGACCGCACCAGCGACACAGAGATCG17160.11233388038274691No Hit
TGTGGAGATGGTCGCATTGCTGAGACCGCACCAGCGACACAAGATCGGAA15300.10015783041119045No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)