FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1949838
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTA1743928.943922520742749TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATATCGTA43710.22417246971286847TruSeq Adapter, Index 5 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT32660.167501094962761No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG25560.1310878134491173No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA21820.11190673276446556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)