FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217102
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTA12701110.435526356870666TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATATCGTA65110.5349592721070214TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG28760.23629901191518868No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATTTCGTA15640.12850196614581194TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA14890.12233978746234908No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT13800.11338408777571642No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT13130.10787920815182293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)