FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550618
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATCCTCATCTCGTA18888212.181078769884007TruSeq Adapter, Index 16 (97% over 39bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT87810.5662903435920388No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG73580.4745204815112426No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA42380.27331038334393126No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA40280.25976739596728526No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT34690.22371725337897536No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT31800.20507952313206734No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA31680.20430563813911615No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATCCTCATATCGTA30310.19547045113625663TruSeq Adapter, Index 16 (97% over 39bp)
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA26790.17276982467635485No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA26590.1714800163547695No Hit
TGTATCGCCAATCACACCGGTTGAGACCGCACCAGCGACACAAGATCGGA25720.16586935015587334No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT23240.14987572696821524No Hit
CTGTGTAGCAGAAGACAGGGCATGAGACCGCACCAGCGACACAGAGATCG22870.1474895815732824No Hit
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT22660.14613528283561777No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCCATCCTCATCTCGTATGC20840.1343980271091913TruSeq Adapter, Index 16 (97% over 37bp)
TGTGGAGATGGTCGCATTGCTGAGACCGCACCAGCGACACAAGATCGGAA20040.12923879382284997No Hit
TGTGGCAAACCACTGGTGTCTGAGACCGCACCAGCGACACAAGATCGGAA17930.11563131603012478No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)