FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550618
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC19414412.520427339293109Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT83340.5374631276046067No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG69690.449433709656408No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA40090.25854207806177926No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA38920.25099669938050506No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT32990.21275388264550005No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT31560.20353175314616495No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA30540.19695373070607977No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA25930.16722364889353794No Hit
TGTGTCGCTGGTGCGGTCTCACTCCCTACTCGGGCCACACAAGATCGGAA25790.1663207830684282No Hit
TGTGTCGCTGGTGCGGTCTCAACCGGTGTGATTGGCGATACAAGATCGGA24970.16103256894992835No Hit
TGTGTCGCTGGTGCGGTCTCAGCGGTAAAACCAGTATTTCGTCACAAGAT21810.14065359746888015No Hit
TGTGTCGCTGGTGCGGTCTCATTTGTTTTTGTTTCCACTTGACACAAGAT21600.13929929873121555No Hit
CTGTGTCGCTGGTGCGGTCTCATGCCCTGTCTTCTGCTACACAGAGATCG21130.13626824917549002No Hit
TGTGTCGCTGGTGCGGTCTCAGACACCAGTGGTTTGCCACAAGATCGGAA16640.10731205235589938No Hit
TGTGTCGCTGGTGCGGTCTCAGCAATGCGACCATCTCCACAAGATCGGAA16060.10357160822330193No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)