FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086699
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATCTCGTA902238.302483024278112TruSeq Adapter, Index 2 (97% over 36bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT32240.29667828902023463No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG22200.20428840000772985No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA20840.19177343496221125No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATATCGTA19460.1790744263130821TruSeq Adapter, Index 2 (97% over 36bp)
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA14270.13131511117613986No Hit
TGTGTCGCTGGTGCGGTCTCATTGGAGGGGTCTCTGCCACAAGATCGGAA14040.1291986097346183No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA13310.12248101820283262No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT13260.12202090919380619No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)