FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306526
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC275822.111094612736371Illumina Single End PCR Primer 1 (98% over 50bp)
NTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC36480.27921373168233926No Hit
NTGCTGTCACCTATTTCTTTGACCTCCACCAACCCTGGGAAATTGCTGCT17560.13440222391288043No Hit
NTGTTTTCAGCCTGGTTCATTTGGTACAAGTGACTCAGAAAATGGCCTTT16390.1254471782421475No Hit
NTCCAAGGACCGCAGACTTCTGCTGAAGTGTACCGCATCCTCAAACAGAA15420.11802290960914669No Hit
NCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG14300.10945055819784683No Hit
NTGGGAGGTGCAGAACAGGACAGACCAGCGGGGTGGCATAGAGAAAGTCC14080.10776670345634147No Hit
NTGCCCTCCTGTTTCGATTACGTGATGCACTTTCTGACTGTGTTCTGGAA13660.10455207167710402No Hit
NTCCCAACTTACACCGGCTCCATCTACCCCACCGTGAAGCCCTACCACTA13250.10141397874975316No Hit
NTCTCCGGATACTCGAAGATAAGCCACACCTGGCGCTGGAACTCGTTGCG13210.10110782334220673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)